@phdthesis{oai:ir.kagoshima-u.ac.jp:00001992,
 author = {Md. Mizanur Rahim Khan and モハマド ミザヌル ラヒム カーン},
 month = {2016-10-27, 2016-10-27},
 note = {学位論文の要旨, 学位論文本文, Eggplant (S. melongena L.) is a widely cultivated and economically importantvegetable crop in many countries all over the world. In hybrid seed production ofeggplant, the undesirable selfing and the necessity of labor-intensive manualemasculation (removal of anthers) are the two main quandaries. Incorporation of malesterile character in female parent lines could obviate emasculation in the hybridizationprocess thus reducing the time, labor and cost for the hybrid seed production ofeggplant. Male sterile character is also useful for seedless fruit production of eggplant.Seedless fruits of eggplant are generally more appetizing than seeded ones andpreferred by consumers. It is strongly desired to widen the range of male sterilitysystems for their practical uses, such as hybrid seed production and seedless fruitproduction, in eggplant. Therefore, the purpose of this study is to investigate thepossibility of developing male sterile lines of eggplant (Solanum melongena L.)utilizing the cytoplasms of wild Solanum species. The cytoplasms of S. anguivi and S.kurzii in section Oliganthes and S. virginianum in section Melongena were utilized todevelop cytoplasm substitution lines of eggplant by continuous backcross methodusing eggplant as a nucleus donor and wild species as cytoplasm donor. The cytoplasm of S. anguivi was successfully substituted for that of eggplant.Pollen non-formation type sterility induced in eggplant by the cytoplasm of S. anguivi.The anthers of the pollen non-formation type plants were completely devoid of pollengrains and no meiosis was detected. From the segregation of the pollen formationability of the backcross progenies, it is assumed that a single dominant fertilityrestoring gene controls the pollen formation of the S. melongena with the cytoplasm of S. anguivi. High fruit set percentage, moderately high number of seeds per fruit andhigh seed germination rate found in the backcross progenies indicate high seedfertility of this male sterile line. The present results indicate that the cytoplasm of S.anguivi is useful for inducing male sterility in eggplant.The cytoplasm of S. kurzii was successfully substituted for that of eggplant.
Another indehiscent type functional male sterility was found in backcross progenies.This anther indehiscent character was induced by disharmony between the cytoplasmof S. kurzii and the nucleus of S. melongena. Pollen fertility in all backcross progenieswas lower than those of the parental species. Seed fertility was generally high in allthe backcross progenies examined. The present results indicate that the cytoplasm of S.kurzii is useful for inducing male sterility in eggplant.The cytoplasm of S. virginianum was utilized to substitute for that of eggplantby continuous backcross method. Backcross progenies carried recombinant cpDNA ofthe parents and maternal mtDNA. Anther indehiscent type of male sterility wasexpressed in backcross progenies. This anther indehiscent character is indicated to bea functional male sterility induced by disharmony between the cytoplasmic genes of S.virginianum and the nuclear genes of S. melongena. Pollen fertility in all backcrossprogenies was quite lower than those of the parental species. Seed fertility was high inall the backcross progenies examined. The present results indicate that the cytoplasmof S. virginianum is useful for inducing male sterility in eggplant.Anther culture was performed to produce pure lines of S. virginianum inducedCMS line of eggplant. From a total of 360 cultured anthers a single plantlet wasregenerated. The regenerated plant has both haploid (2n = 12) and diploid (2n = 24)cells in the root tips which indicate that the regenerated plant was originated from themicrospore and chromosome doubling may go on spontaneously. This study revealed that anther culture is useful to develop pure lines of S. virginianum induced CMS lineof eggplant.This study demonstrated that it is possible to develop male sterile lines ofeggplant with the cytoplasms of wild Solanum species, S. anguivi, S. kurzii and S.virginianum and that anther culture is useful to develop pure lines of S. virginianuminduced CMS line of eggplant. The male sterile lines of eggplant developed in thisstudy are potentially valuable for simplifying and reducing the time, labor and cost forthe production of F1 hybrid seeds and seedless fruits of eggplant., 連合農学研究科博士論文(農学) ; 学位取得日: 平成21年3月16日},
 school = {鹿児島大学},
 title = {Development of male sterile lines of eggplant (Solanum melongena L.) utilizing the cytoplasms of wild Solanum species},
 year = {}
}