{"created":"2023-09-26T07:48:43.035839+00:00","id":2000009,"links":{},"metadata":{"_buckets":{"deposit":"e5a04223-7a8e-4217-b617-7ffb714157b1"},"_deposit":{"created_by":18,"id":"2000009","owner":"29","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000009"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:02000009","sets":["57:86"]},"author_link":["143843"],"control_number":"2000009","item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2023-08-21","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2023-08-30"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(歯学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Dental Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文要約, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Background\nMaxillary/mandibular bone marrow-derived mesenchymal stem cells (MBMSCs) exhibit a unique property of lower adipogenic potential than other bone marrow-derived MSCs. However, the molecular mechanisms regulating the adipogenesis of MBMSCs remain unclear. This study aimed to explore the roles of mitochondrial function and reactive oxygen species (ROS) in regulating the adipogenesis of MBMSCs.\n\nMethods and results\nMBMSCs exhibited significantly lower lipid droplet formation than iliac BMSCs (IBMSCs). Moreover, the expression levels of CCAAT/enhancer-binding protein β (C/EBPβ), C/EBPδ, and early B cell factor 1 (Ebf-1), which are early adipogenic transcription factors, and those of peroxisome proliferator-activated receptor-γ (PPARγ) and C/EBPα, which are late adipogenic transcription factors, were downregulated in MBMSCs compared to those in IBMSCs. Adipogenic induction increased the mitochondrial membrane potential and mitochondrial biogenesis in MBMSCs and IBMSCs, with no significant difference between the two cell types; however, intracellular ROS production was significantly enhanced only in IBMSCs. Furthermore, NAD(P)H oxidase 4 (NOX4) expression was significantly lower in MBMSCs than in IBMSCs. Increased ROS production in MBMSCs by NOX4 overexpression or treatment with menadione promoted the expression of early adipogenic transcription factors but did not induce that of late adipogenic transcription factors or lipid droplet accumulation.\n\nConclusions\nThese results suggest that ROS may be partially involved in the process of MBMSC adipogenic differentiation from undifferentiated cells to immature adipocytes. This study provides important insights into the tissue-specific properties of MBMSCs.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Ikeda Nao, Ishii Masakazu, Miyata Haruka, Nishi Yasuhiro, Suehiro Fumio, Komabashiri Naohiro, Sakurai Tomoaki & Nishimura Masahiro\nRole of reactive oxygen species (ROS) in the regulation of adipogenic differentiation of human maxillary/mandibular bone marrow-derived mesenchymal stem cells\nMolecular Biology Reports (2023) 50:5733–5745\nhttps://doi.org/10.1007/s11033-023-08528-9","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s11033-023-08528-9","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第709号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"【指導教員:西村正宏】要約公表の理由: 多重公表を禁止するジャーナルへの投稿論文であるため"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"【学位申請日】2023-07-05"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"池田, 菜緒","creatorNameLang":"ja"},{"creatorName":"イケダ, ナオ","creatorNameLang":"ja-Kana"},{"creatorName":"Ikeda, Nao","creatorNameLang":"en"}],"familyNames":[{"familyName":"池田","familyNameLang":"ja"},{"familyName":"イケダ","familyNameLang":"ja-Kana"},{"familyName":"Ikeda","familyNameLang":"en"}],"givenNames":[{"givenName":"菜緒","givenNameLang":"ja"},{"givenName":"ナオ","givenNameLang":"ja-Kana"},{"givenName":"Nao","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"143843","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-06-23"}],"displaytype":"detail","fileDate":[{"fileDateType":"Collected","fileDateValue":"2023-08-21"}],"filename":"Digest_池田_菜緒_ISK709_2023.pdf","filesize":[{"value":"633 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"Digest_池田_菜緒_ISK709_2023.pdf","objectType":"summary","url":"https://ir.kagoshima-u.ac.jp/record/2000009/files/Digest_池田_菜緒_ISK709_2023.pdf"},"version_id":"8b8266ac-7de3-4131-9446-723d6dbe992c"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-31"}],"displaytype":"detail","fileDate":[{"fileDateType":"Collected","fileDateValue":"2023-08-21"}],"filename":"Abstract_池田_菜緒_4519810042_2023.pdf","filesize":[{"value":"159 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"Abstract_池田_菜緒_4519810042_2023","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/2000009/files/Abstract_池田_菜緒_4519810042_2023.pdf"},"version_id":"85b78512-f0bf-4ded-bd76-c69e9be59e4f"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-09-26"}],"displaytype":"detail","fileDate":[{"fileDateType":"Collected","fileDateValue":"2023-08-21"}],"filename":"Result_IKEDA_Nao_isk_709 .pdf","filesize":[{"value":"142 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/2000009/files/Result_IKEDA_Nao_isk_709 .pdf"},"version_id":"be35a523-5a62-4cc7-b612-e21f8f43e472"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-09-26"}],"displaytype":"detail","fileDate":[{"fileDateType":"Collected","fileDateValue":"2023-08-21"}],"filename":"Comments_IKEDA_Nao_isk_709 .pdf","filesize":[{"value":"53 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/2000009/files/Comments_IKEDA_Nao_isk_709 .pdf"},"version_id":"43f202eb-c245-4223-9ba2-a8aed31ca5b3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Mesenchymal stem cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Maxillary/mandibular bone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Iliac bone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Adipogenic differentiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Reactive oxygen species","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"},{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Role of reactive oxygen species (ROS) in the regulation of adipogenic differentiation of human maxillary/mandibular bone marrow-derived mesenchymal stem cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Role of reactive oxygen species (ROS) in the regulation of adipogenic differentiation of human maxillary/mandibular bone marrow-derived mesenchymal stem cells","subitem_title_language":"en"},{"subitem_title":"ヒト顎骨骨髄由来間葉系幹細胞の脂肪分化制御における活性酸素種（ROS）の役割","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-09-26"},"publish_date":"2023-09-26","publish_status":"0","recid":"2000009","relation_version_is_last":true,"title":["Role of reactive oxygen species (ROS) in the regulation of adipogenic differentiation of human maxillary/mandibular bone marrow-derived mesenchymal stem cells"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2025-06-23T05:48:05.520178+00:00"}