{"created":"2023-09-26T08:00:10.780608+00:00","id":2000010,"links":{},"metadata":{"_buckets":{"deposit":"c512995f-b109-4674-8b4f-2f04f278d1e4"},"_deposit":{"created_by":18,"id":"2000010","owner":"29","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000010"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:02000010","sets":["57:86"]},"author_link":["143844"],"control_number":"2000010","item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2023-08-21","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2023-08-30"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(歯学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Dental Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Introduction: The trigeminal nerve conveys delicate sensations such as warmth, pain, and tactile pressure in the oral and facial regions, and most trigeminal afferent cell bodies are located in the trigeminal ganglion. Our previous study has shown that sensations in trigeminal nerve innervated areas, specifically in the maxillofacial region, exhibit diurnal variation and that sensitivity changes time-dependently. In this study, we aimed to clarify the rhythm of expression of clock gene in the trigeminal ganglion of mice to elucidate the mechanism of circadian regulation in the same area.\nMethods: Immunohistochemistry examined the expression of the PER2 protein in the suprachiasmatic nucleus and trigeminal ganglion of wild-type mice. To measure gene expression as bioluminescence, PERIOD2::LUCIFERASE knock-in (PER2::LUC) mice were used. Unilateral trigeminal ganglion and brain sections including the suprachiasmatic nucleus were incubated ex vivo. Bioluminescence levels were then measured using a highly sensitive photodetector. The same experiments were then conducted with Cry1 gene-deficient (Cry1−/−) or Cry2 gene-deficient (Cry2−/−) mice.\nResults: In the trigeminal ganglion, immunohistochemistry localized PER2 protein expression within the neuronal cell body. Mouse trigeminal ganglion ex vivo tissues showed distinct circadian oscillations in PER2::LUC levels in all genotypes, wild-type, Cry1−/−, and Cry2−/−. The period was shorter in the trigeminal ganglion than in the suprachiasmatic nucleus; it was shorter in Cry1−/− and longer in Cry2−/− mice than in the wild-type mice.\nConclusion: The expression of Per2 in neurons of the trigeminal ganglion in ex vivo culture and the oscillation in a distinct circadian rhythm suggests that the trigeminal ganglion is responsible for the relay of sensory inputs and temporal gating through autonomous circadian oscillations.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Yukie Shirakawa, Sachi N. Ohno, Kanae A. Yamagata, Eriko Kuramoto, Yoshiaki Oda, Takahiro J. Nakamura, Wataru Nakamura and Mitsutaka Sugimura (2023) \nCircadian rhythm of PERIOD2::LUCIFERASE expression in the trigeminal ganglion of mice\nFrontiers in Neuroscience 17:1142785.\nhttps://doi.org/10.3389/fnins.2023.1142785","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"dissertation_number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第710号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Frontiers","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"【指導教員:杉村光隆】"}]},"item_5_text_47":{"attribute_name":"item_5_text_47","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2023-05-10"}]},"item_5_version_type_14":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"白川, 由紀恵","creatorNameLang":"ja"},{"creatorName":"シラカワ, ユキエ","creatorNameLang":"ja-Kana"},{"creatorName":"Shirakawa, Yukie","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{"nameIdentifier":"143844","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-09-26"}],"displaytype":"detail","filename":"Diss_白川_由紀恵_ISK710_2023.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"label":"Diss_白川_由紀恵_ISK710_2023","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/2000010/files/Diss_白川_由紀恵_ISK710_2023.pdf"},"version_id":"c1411357-b474-4e1f-81aa-fd0859f52793"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-09-26"}],"displaytype":"detail","filename":"Abstract_白川_由紀恵_4520810125_2023.pdf","filesize":[{"value":"168 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"Abstract_白川_由紀恵_4520810125_2023","objectType":"abstract","url":"https://ir.kagoshima-u.ac.jp/record/2000010/files/Abstract_白川_由紀恵_4520810125_2023.pdf"},"version_id":"90fe6c86-7743-4a5c-803a-6e70d567878f"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-09-26"}],"displaytype":"detail","filename":"Result_SHIRAKAWA_Yukie_isk_710.pdf","filesize":[{"value":"98 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/2000010/files/Result_SHIRAKAWA_Yukie_isk_710.pdf"},"version_id":"57a2cdf6-25f0-482f-b125-75a59a00c721"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-09-26"}],"displaytype":"detail","filename":"Comments_SHIRAKAWA_Yukie_isk_710.pdf","filesize":[{"value":"55 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/2000010/files/Comments_SHIRAKAWA_Yukie_isk_710.pdf"},"version_id":"d3597165-3bc6-4748-a6b0-598849a6d29c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Circadian rhythm","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Suprachiasmatic nucleus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Trigeminal ganglion","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PER2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cryptochrome","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"},{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Circadian rhythm of PERIOD2::LUCIFERASE expression in the trigeminal ganglion of mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Circadian rhythm of PERIOD2::LUCIFERASE expression in the trigeminal ganglion of mice","subitem_title_language":"en"},{"subitem_title":"マウスの三叉神経節におけるPERIOD2::LUCIFERASE発現の概日リズム","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"18","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-09-26"},"publish_date":"2023-09-26","publish_status":"0","recid":"2000010","relation_version_is_last":true,"title":["Circadian rhythm of PERIOD2::LUCIFERASE expression in the trigeminal ganglion of mice"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-10-31T08:51:43.121445+00:00"}