{"created":"2023-09-28T01:48:14.511073+00:00","id":2000018,"links":{},"metadata":{"_buckets":{"deposit":"3ccc4a6a-aa73-43f1-942b-449a35c6d332"},"_deposit":{"created_by":29,"id":"2000018","owner":"29","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"2000018"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:02000018","sets":["57:86"]},"author_link":["143851"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2023-09-15","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2023-09-21"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(歯学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Dental Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Dedifferentiated fat cells (DFATs) isolated from mature adipocytes have a multilineage differentiation capacity similar to mesenchymal stem cells and are considered as promising source of cells for tissue engineering. Bone morphogenetic protein 9 (BMP9) and low-intensity pulsed ultrasound (LIPUS) have been reported to stimulate bone formation both in vitro and in vivo. However, the combined effect of BMP9 and LIPUS on osteoblastic differentiation of DFATs has not been studied. After preparing DFATs from mature adipose tissue from rats, DFATs were treated with different doses of BMP9 and/or LIPUS. The effects on osteoblastic differentiation were assessed by changes in alkaline phosphatase (ALP) activity, mineralization/calcium deposition, and expression of bone related genes; Runx2, osterix, osteopontin. No significant differences for ALP activity, mineralization deposition, as well as expression for bone related genes were observed by LIPUS treatment alone while treatment with BMP9 induced osteoblastic differentiation of DFATs in a dose dependent manner. Further, co-treatment with BMP9 and LIPUS significantly increased osteoblastic differentiation of DFATs compared to those treated with BMP9 alone. In addition, upregulation for BMP9-receptor genes was observed by LIPUS treatment. Indomethacin, an inhibitor of prostaglandin synthesis, significantly inhibited the synergistic effect of BMP9 and LIPUS co-stimulation on osteoblastic differentiation of DFATs. LIPUS promotes BMP9 induced osteoblastic differentiation of DFATs in vitro and prostaglandins may be involved in this mechanism.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Fumiaki Setoguchi, Kotaro Sena, Kazuyuki Noguchi\nLow-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells\nInternational Journal of Stem Cells 2023;16:406-414\nhttps://doi.org/10.15283/ijsc23027","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第713号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Korean Society for Stem Cell Research","subitem_publisher_language":"en"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"【指導教員:野口和行】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2023-07-05"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"瀬戸口, 史晃","creatorNameLang":"ja"},{"creatorName":"セトグチ, フミアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Setoguchi, Fumiaki","creatorNameLang":"en"}],"familyNames":[{"familyName":"瀬戸口","familyNameLang":"ja"},{"familyName":"セトグチ","familyNameLang":"ja-Kana"},{"familyName":"Setoguchi","familyNameLang":"en"}],"givenNames":[{"givenName":"史晃","givenNameLang":"ja"},{"givenName":"フミアキ","givenNameLang":"ja-Kana"},{"givenName":"Fumiaki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"143851","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-06-24"}],"displaytype":"detail","fileDate":[{"fileDateType":"Collected","fileDateValue":"2023-09-15"}],"filename":"Diss_SETOGUCHI_Fumiaki_ISK713_2023.pdf","filesize":[{"value":"511.3 KB"}],"format":"application/pdf","licensetype":"license_3","mimetype":"application/pdf","url":{"label":"Diss_SETOGUCHI_Fumiaki_ISK713_2023.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/2000018/files/Diss_SETOGUCHI_Fumiaki_ISK713_2023.pdf"},"version_id":"e9fff014-7c28-4a79-ae40-954797ababf5"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-06-24"}],"displaytype":"detail","fileDate":[{"fileDateType":"Collected","fileDateValue":"2023-09-15"}],"filename":"Abstract_SETOGUCHI_Fumiaki_4519810196_2023.pdf","filesize":[{"value":"121.5 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cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bone morphogenetic protein 9","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Low-intensity pulsed ultrasound","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Prostaglandins","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells","subitem_title_language":"en"},{"subitem_title":"低出力超音波パルスはBMP9により誘導されたラット脱分化脂肪細胞の骨芽細胞様分化を促進する","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"29","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-09-28"},"publish_date":"2023-09-28","publish_status":"0","recid":"2000018","relation_version_is_last":true,"title":["Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2025-06-24T01:49:41.827460+00:00"}