{"created":"2025-07-07T01:15:01.300574+00:00","id":2001251,"links":{},"metadata":{"_buckets":{"deposit":"c3ce87fb-5854-40d7-bc76-b76681d97411"},"_deposit":{"created_by":29,"id":"2001251","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"2001251"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:02001251","sets":["57:86"]},"author_link":["144778"],"item_5_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2025-06-05","subitem_date_issued_type":"Collected"}]},"item_5_date_granted_54":{"attribute_name":"学位授与年月日 ","attribute_value_mlt":[{"subitem_dategranted":"2025-06-12"}]},"item_5_degree_grantor_53":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"鹿児島大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"17701","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_5_degree_name_42":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"},{"subitem_degreename":"Doctor of Philosophy in Medical Science","subitem_degreename_language":"en"}]},"item_5_description_17":{"attribute_name":"ファイル（説明）","attribute_value_mlt":[{"subitem_description":"博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_5_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Purpose　The lack of specific autopsy findings for carbon dioxide (CO2) intoxication hinders the determination of cause of death based on autopsy findings alone. In addition, when death occurs in a space is filled with CO2 or other gases, the cause of death must be distinguished between intoxication and asphyxia due to oxygen deficiency, which also has no specific autopsy findings. In this study, we aimed to identify diagnostic markers of mRNA expression in the brainstem that indicate cause of death in cases of suspected CO2 intoxication.\nMethods　Mouse models of CO2 intoxication (composition of ambient gases at 70% CO2, 20% O2, and 10% N2) and asphyxia due to oxygen deficiency (5% O2, 95% N2) were used to identify mRNA markers specific to intoxication or asphyxia.\nResults　Using RNA-Sequence analysis, we identified 7 candidate genes for qRT-PCR analysis: Acid-sensing ion channel 4 (Asic4), Early growth response protein 1 (Egr1), Neurogranin (Nrgn), Opioid receptor delta 1 (Oprd1), Semaphorin 3f (Sema3f), Transthyretin (Ttr), and Tryptophan hydroxylase 2 (Tph2). We observed a significant increase of Nrgn mRNA expression in the brainstem of CO2 intoxication and a significant increase of Ttr mRNA expression in the brainstem of asphyxia due to oxygen deficiency.\nConclusion　Assays for the expression of Nrgn and Ttr in the human brainstem may assist in the diagnosis/differential diagnosis of CO2 intoxication and asphyxia due to oxygen deficiency, respectively.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Masahiko Yatsushiro, Midori Katsuyama, Takuma Nakamae, Kotomi Imahara, Machiko Miyamoto & Takahito Hayashi\nNew molecular markers to differentiate carbon dioxide intoxication from asphyxia due to oxygen deficiency\nForensic Sci Med Pathol (2025)\nhttps://doi.org/10.1007/s12024-025-00981-1","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"The version of record of this article, first published in Forensic Science, Medicine and Pathology, is available online at Publisher’s website: https://doi.org/10.1007/s12024-025-00981-1","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_5_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲総研第791号"}]},"item_5_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"},{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"}]},"item_5_text_44":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"【指導教員:林敬人】"}]},"item_5_text_47":{"attribute_name":"date.appl","attribute_value_mlt":[{"subitem_text_value":"【学位申請日】2025-04-09"}]},"item_5_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"八代, 正彦","creatorNameLang":"ja"},{"creatorName":"Yatsushiro, Masahiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"144778","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-10-27"}],"displaytype":"detail","fileDate":[{"fileDateType":"Collected","fileDateValue":"2025-06-05"}],"filename":"Diss_YATSUSHIRO_Masahiko_ISK791_2025.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"label":"Diss_YATSUSHIRO_Masahiko_ISK791_2025.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/2001251/files/Diss_YATSUSHIRO_Masahiko_ISK791_2025.pdf"},"version_id":"64cea730-38b9-461f-8cc8-65e0ca6c1bdb"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-10-27"}],"displaytype":"detail","fileDate":[{"fileDateType":"Collected","fileDateValue":"2025-06-05"}],"filename":"Abstract_YATSUSHIRO_Masahiko_4521800080_2025.pdf","filesize":[{"value":"27.6 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KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"Result_YATSUSHIRO_Masahiko_isk_791.pdf","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/2001251/files/Result_YATSUSHIRO_Masahiko_isk_791.pdf"},"version_id":"ac1ac612-cf75-418d-88ac-6c62f400dd80"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-07-02"}],"displaytype":"detail","fileDate":[{"fileDateType":"Collected","fileDateValue":"2025-06-05"}],"filename":"Comments_YATSUSHIRO_Masahiko_isk_791.pdf","filesize":[{"value":"54.3 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"Comments_YATSUSHIRO_Masahiko_isk_791.pdf","objectType":"other","url":"https://ir.kagoshima-u.ac.jp/record/2001251/files/Comments_YATSUSHIRO_Masahiko_isk_791.pdf"},"version_id":"8288f914-55b6-4a51-bb79-5aa9e4f400b1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Carbon dioxide intoxication","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Asphyxia due to oxygen deficiency","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Molecular markers","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Postmortem differential diagnosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"New molecular markers to differentiate carbon dioxide intoxication from asphyxia to oxygen deficiency","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"New molecular markers to differentiate carbon dioxide intoxication from asphyxia to oxygen deficiency","subitem_title_language":"en"},{"subitem_title":"二酸化炭素中毒及び酸素欠乏による窒息の死因鑑別のための新規分子マーカー","subitem_title_language":"ja"}]},"item_type_id":"5","owner":"29","path":["86"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-07-07"},"publish_date":"2025-07-07","publish_status":"0","recid":"2001251","relation_version_is_last":true,"title":["New molecular markers to differentiate carbon dioxide intoxication from asphyxia to oxygen deficiency"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2025-10-27T08:33:31.393811+00:00"}