{"created":"2025-07-07T07:15:54.507854+00:00","id":2001255,"links":{},"metadata":{"_buckets":{"deposit":"780fa895-0a92-4301-aa25-b5eb8b8b901b"},"_deposit":{"created_by":29,"id":"2001255","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"2001255"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:02001255","sets":["228:230:1750985049716","57:58:59"]},"author_link":["144809","132085","144813","56603","144816","1192","1199"],"control_number":"2001255","item_7_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"28","bibliographicPageStart":"22","bibliographicVolumeNumber":"76","bibliographic_titles":[{"bibliographic_title":"鹿児島大学医学雑誌","bibliographic_titleLang":"ja"},{"bibliographic_title":"Medical journal of Kagoshima University","bibliographic_titleLang":"en"}]}]},"item_7_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2024-12","subitem_date_issued_type":"Issued"}]},"item_7_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"In chronic wounds such as bedsores, bacterial infection prolongs wound healing, and biofilms are a particularly important inhibitory factor in wound healing. Therefore, wound management requires inhibition of biofilm formation, and research is being conducted to develop products with anti-biofilm properties. Silk is composed of two types of proteins, fibroin and sericin, and it has been reported that fibroin promotes wound healing and sericin has antibacterial activity. Therefore, we wondered whether sericin could inhibit biofilm formation and investigated the inhibitory effect of sericin on thebiofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from wounds in vitro. Two MRSA strains with high biofilm forming ability and a standard strain were used. Sericin was extracted from silk by high-pressure steam sterilization. Sericin was added to the biofilm forming process of MRSA strains and its inhibitory effect on biofilm formation was evaluated. Morphological observation showed that the pigment in the stained biofilm was attenuated. Biofilm assay using a microtiter plate showed that the mean biofilm index was significantly inhibited by 66.1% in the sericin 250 μg/mL group compared to the control group (0.417 vs. 1.231, p < 0.01). These results show that silk sericin has an inhibitory effect on the biofilm formation by MRSA strains. Sericin contains many positively charged amino acids with hydrophilic amino acid residues, which are easy to combine with the negatively charged bacterial surface. The feature of molecular structure was thought to be related to its anti-biofilm properties.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"褥瘡など慢性創傷において細菌感染は創傷治癒を遷延化させるが，特に創傷治癒の重要な阻害要因としてバイオフィルムの存在がある．そのため創傷管理では，バイオフィルムの形成を阻害することが求められ，抗バイオフィルム作用を有する製品開発が進められている．シルクは，フィブロインとセリシンの２種類のタンパク質で構成され，フィブロインは創傷治癒を促進し，セリシンは抗菌作用を有するとの報告がある．そこで，慢性創傷の難治化する要因であるバイオフィルムの形成をセリシンによって阻害できないかと考え，創傷から分離されたメチシリン耐性黄色ブドウ球菌(methicillin-resistant Staphylococcus aureus: MRSA) 株のバイオフィルム形成能に対する影響をin vitroで検討した．MRSA株は，高バイオフィルム形成能を有する臨床分離株２株と標準株を用いた．シルクは高圧蒸気滅菌法でフィブロインとセリシンに分離させてセリシンを抽出した．バイオフィルム形成過程でセリシンを加え，バイオフィルム形成に対する阻害効果を形態学的に観察するとともに，マイクロタイタープレートを用いたバイオフィルムアッセイでbiofilmindex (BI)を測定した．形態学的観察では染色したバイオフィルムの色素が減弱することを確認した．バイオフィルムアッセイでは，最終濃度250 μg/mLのセリシン添加群が対照群に比べて平均BIが66.1％低下しており(0.417 vs. 1.231, p < 0.01)，セリシンがMRSAによるバイオフィルム形成を阻害することが明らかになった．セリシンは正に荷電した親水性アミノ酸残基をもつアミノ酸を多く含有し，負に荷電している細菌表面と結合しやすいことが予想され，その分子構造がバイオフィルム形成阻害効果と関連していると考えられた．","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_7_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03685063","subitem_source_identifier_type":"PISSN"}]},"item_7_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00040104","subitem_source_identifier_type":"NCID"}]},"item_7_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西帯野, 智代","creatorNameLang":"ja"},{"creatorName":"NISHIOBINO, Tomoyo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"144809","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"馬場, 淳徳","creatorNameLang":"ja"},{"creatorName":"BABA, Atsunori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"132085","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"福重, 智子","creatorNameLang":"ja"},{"creatorName":"FUKUSHIGE, Tomoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"144813","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藺牟田, 直子","creatorNameLang":"ja"},{"creatorName":"IMUTA, Naoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"56603","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中村, 政敏","creatorNameLang":"ja"},{"creatorName":"NAKAMURA, Masatoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"144816","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西, 順一郎","creatorNameLang":"ja"},{"creatorName":"NISHI, Junichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1192","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"金蔵, 拓郎","creatorNameLang":"ja"},{"creatorName":"KANEKURA, Takuro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1199","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2025-07-07"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"2024-12"}],"filename":"Med._J._Kagoshima_Univ_76_p22-28.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"Med._J._Kagoshima_Univ_76_p22-28.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/2001255/files/Med._J._Kagoshima_Univ_76_p22-28.pdf"},"version_id":"b8d5a6c0-e671-4177-8823-bb322332e7ab"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"chronic wound","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"biofilm","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"methicillin-resistant Staphylococcus aureus (MRSA)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"silk","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sericin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"シルクセリシンのメチシリン耐性黄色ブドウ球菌バイオフィルム形成抑制効果","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"シルクセリシンのメチシリン耐性黄色ブドウ球菌バイオフィルム形成抑制効果","subitem_title_language":"ja"},{"subitem_title":"Inhibitory effect of silk sericin on biofilm formation by methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates","subitem_title_language":"en"}]},"item_type_id":"7","owner":"29","path":["1750985049716","59"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-07-07"},"publish_date":"2025-07-07","publish_status":"0","recid":"2001255","relation_version_is_last":true,"title":["シルクセリシンのメチシリン耐性黄色ブドウ球菌バイオフィルム形成抑制効果"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2025-07-07T07:37:52.077048+00:00"}