{"created":"2026-01-14T08:53:54.039376+00:00","id":2002126,"links":{},"metadata":{"_buckets":{"deposit":"207f7e47-73a1-437c-96b5-ba4608ad0b85"},"_deposit":{"created_by":29,"id":"2002126","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"2002126"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:02002126","sets":["117:118","228:1745312197915:1764639438513"]},"author_link":["144527","144718","144646"],"item_6_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2025-12-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"36","bibliographicPageStart":"31","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"Ichthy, Natural History of Fishes of Japan","bibliographic_titleLang":"en"}]}]},"item_6_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2025-12-25","subitem_date_issued_type":"Issued"}]},"item_6_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Cobitis striata Ikeda, 1936 comprises three subspecies, among which Cobitis striata hakataensis Nakajima, 2012 and Cobitis striata fuchigamii Nakajima, 2012 are geographically restricted to northern Kyushu, Japan. Cobitis striata striata Ikeda, 1936 is distributed in parts of Honshu, Shikoku, and Kyushu. These subspecies are listed as critically endangered (C. s. hakataensis), endangered (C. s. fuchigamii), and vulnerable (C. s. striata) in the Red Data Book of Japan. In May 2025, a previously unrecorded haplotype closely matching C. s. striata or C. s. fuchigamii was detected in the river flowing into Hakata Bay. We developed a genetic screening method using PCR–restriction fragment length polymorphism (PCR-RFLP) targeting mitochondrial cyt b gene (1020 bp). Enzyme digestion revealed distinct band patterns for C. s. hakataensis (800, 210, 100 bp), C. s. striata / C. s. fuchigamii (900, 210 bp), C. kaibarai (800, 180, 100, 30 bp), and C. matsubarae (800, 310 bp). Rare polymorphisms were observed in some cases, indicating that PCR-RFLP is suitable for first-tier screening but should be combined with morphology and sequencing for final identification.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学総合研究博物館","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University Museum","subitem_publisher_language":"en"}]},"item_6_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.34583/ichthy.62.0_31","subitem_relation_type_select":"DOI"}}]},"item_6_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2025 鹿児島大学総合研究博物館 この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。","subitem_rights_language":"ja","subitem_rights_resource":"https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja"}]},"item_6_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2435-7715","subitem_source_identifier_type":"EISSN"}]},"item_6_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"永江, 栞奈","creatorNameLang":"ja"},{"creatorName":"Nagae, Kanna","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"144527","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"栗田, 喜久","creatorNameLang":"ja"},{"creatorName":"Kurita, Yoshihisa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"144718","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鬼倉, 徳雄","creatorNameLang":"ja"},{"creatorName":"Onikura, Norio","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"144646","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2026-01-14"}],"displaytype":"detail","fileDate":[{"fileDateType":"Issued","fileDateValue":"2025-12-25"}],"filename":"INHFJ_2025_62_31.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"label":"INHFJ_2025_62_31.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/2002126/files/INHFJ_2025_62_31.pdf"},"version_id":"fe4df186-3da4-4bd9-ad20-c8454f7e0ae5"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"博多湾流入河川におけるナミスジシマドジョウからの未確認ハプロタイプの検出とPCR-RFLP分析による種・亜種判別","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"博多湾流入河川におけるナミスジシマドジョウからの未確認ハプロタイプの検出とPCR-RFLP分析による種・亜種判別","subitem_title_language":"ja"},{"subitem_title":"Detection of unidentified haplotypes from Cobitis striata identification using PCR-RFLP analysis in rivers flowing into Hakata Bay, Japan","subitem_title_language":"en"}]},"item_type_id":"6","owner":"29","path":["118","1764639438513"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2026-01-14"},"publish_date":"2026-01-14","publish_status":"0","recid":"2002126","relation_version_is_last":true,"title":["博多湾流入河川におけるナミスジシマドジョウからの未確認ハプロタイプの検出とPCR-RFLP分析による種・亜種判別"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2026-03-03T13:26:29.808151+00:00"}