@article{oai:ir.kagoshima-u.ac.jp:00002235,
 author = {SAKATA, Taizo and SUAMI, Shojiro and KAKIMOTO, Daiichi},
 journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University},
 month = {2016-10-27},
 note = {The germination of Bacillus subtilis spores was induced sufficiently at 1 mM of L-alanine and at the lower concentration than 1 mM the germination rates increased dependency on the concentration of L-alanine. Optimal pH of the germination at 25 C and 37 C was neutral, while at lower temperature such as 6 C and 14 C it shifted to acid side, pH 5.0. Glycine (at 100 mM), L-cysteine (at 100 mM) and Hg^{2+} (at 10 mM) inhibited the alanineinduced germination remarkably. Effects of chemical treatments on spores varied with the conditions of treatments. Treatments of spores with 0.1 N NaOH at 80 C, 0.1 N HCl at 60 C, 5% sodium lauryl sulfate (SLS) at 60 C and 5% phenol at 60 C broke the capacity of germination completely. The spores by treatment with NaOH at 40 C and SLS at 60 C became lysozyme-sensitive. Treatment with NaOH at 60 C decreased the germination rates, failed to induce histidase during outgrowth and made the spores sensitive to lysozyme but it had little effect on viability and heat resistance.},
 pages = {139--147},
 title = {Factors affecting the germination of Bacillus subtilis spores},
 volume = {24},
 year = {}
}