@article{oai:ir.kagoshima-u.ac.jp:00002337,
 author = {HAYASHI, Seiichi and MORSHED, Monzoor and FUKUYAMA, Keiko and NAKASAKO, Keisuke and OOSHIRO, Zentaro},
 journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University},
 month = {2016-10-27},
 note = {Native glutamate dehydrogenase (GDH) was purified from the eel liver through procedures
including fractionation of mitochondria, absorption chromatography with hydroxyapatite,
ion-exchange chromatography with DEAE-Sephacel, affinity chromatography with
GTP-Sepharose, and gel filtration with Toyopearl HW-55. Molecular weight of native GDH
was 340,000 and the subunit of the GDH consisted of one kind of protein with molecular weight
of 53,000 to 55,000. Isoelectric point of the GDH was pH 5.9. Amino acid composition of the
GDH was analyzed and had a certain similarity to that of bovine liver GDH. The composition of
glycine, histidine, methionine, tryptophan, tyrosine and valine in both GDHs was very
similar.},
 pages = {1--10},
 title = {Purification of Native Glutamate Dehydrogenase from Eel Liver},
 volume = {37},
 year = {}
}