@article{oai:ir.kagoshima-u.ac.jp:00002341,
 author = {大城, 善太郎},
 journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University},
 month = {2016-10-27},
 note = {A rapid method of the colorimetric estimation of the modicum of protein in fish muscle under the use of biuret reagent (modified Sol's reagent) was put under examination, with the following procedure ascertained. After taking 5ml. of sample into a colorimeter tube in which 0.5 to 4.5mg. protein-nitogen was contained, 5ml. of biuret reagent was added. Then, after a well mixing, it was heated for 1 minute at 100°C on a water bath, to accelerate the color development. Although, the maximum color development required 1 minute, the development itself occurred almost immediately. Then, the colorimeter tube was cooled in a running water for about 1 minute. And with the purpose of removing the turbid substances yielded, 1 ml of reagent mixture (20% MgSO_4・7H_2O - 35% ZnSO_4・7H_2O) was added to the above mentioned colored solution. Then, those substances were adsorbed by the colloidal magnesium hydroxide prepared with magnesium sulfate and sodium hydroxide in biuret colored solution, in this case the former was added after being well mixed by the vigorous shaking for a  spell of several seconds. The mixed solution was filtered after 2 minutes standing. The optical density of the filtrate was read at the wave length of 530mμ. By the proposed method, the determination of protein was to be done with comparatively correct accuracy, the error, compared with that of Kjeldahl value, being to be kept within the range of ±2%.},
 pages = {119--124},
 title = {ビューレット試薬による魚肉蛋白質の迅速定量I : 呈色反応の促進と発色液の濁りの除去},
 volume = {6},
 year = {}
}