@article{oai:ir.kagoshima-u.ac.jp:00002343, author = {SAKATA, Taizo}, journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University}, month = {2016-10-27}, note = {Two types of LPS mutants were isolated from a virulent strain, TF7 of Aeromonas hydrophila after UV irradiation. LPS profiles of TF7 and its mutants were revealed by using SDS-PAGE and immunoblotting techniques. One type of the mutants was found to lose O-saccharide chain of LPS completely and concomitantly to change to be serum sensitive. The other type of the mutants displayed to produce less amount of O-side chains than TF7 and to be resistant to rabbit serum conditionally. When the cells were grown on TSA containing SDS (TSA-SDS) or MacConkey agars, the former mutants exhibited higher sensitivity, while the latter turned to be serum sensitive considerably. The parent strain TF7 were not significantly different in respect with serum susceptibility even if grown on TSA-SDS agar. The most distinguishable feature of whole cell protein profiles on SDS-PAGE was observed as the decrease of 49 Kd band concomitantly with increase 41 Kd band for both the parent and mutant strains grown on TSA-SDS agar.}, pages = {147--159}, title = {Serum Sensitivity of a Fish Pathogenic Strain and Its LPS Mutants of Aeromonas hydrophila}, volume = {37}, year = {} }