@misc{oai:ir.kagoshima-u.ac.jp:00002357,
 author = {山口, 昭彦 and Yamaguchi, Akihiko},
 month = {},
 note = {Vesnarinone is a positive inotropic agent that is used to treat for congestive heart failure. Recently，it has been shown to inhibit the growth of several cancer cells such as salivary cancer，pancreatie cancer and gastric cance. Large cell lung cancer is known to be refractory to usual chemotherapy，and new effective therapy for this type oflung cancer is needed. In the present study，I have examined the effect of vesnarinone on large cell lung cancer cell lines. Three large cell lung cancer cell lines (LU65A，LU65B，LU65C) were examined. Growth inhibition of these cells by vesnarinone was assayed with 3H-thymidine
incorporation after 72-hrs culture. Apoptosis induction of LU65A cell was examined by the TUNEL method and DNA fragmentation. Expression of Bcl-2 and Fas antigen in the cell was detected by FACS analysis. Expression of c-myc mRNA was examined by Northern Blotting. Vesnarinone inhibited 
3H-thymidine incorporation in a dose dependent manner at concentrations from 0.1 to 20 μg/ml in all cell lines. TUNEL method and gel electrophoresis showed vesnarinone-induced apoptosis in Lu65A cells Vesnarinone induced expression of Fas antigen and c-myc，but did not affect the expression of Bcl-2 in Lu65A cells. These results suggest that Fas antigen and c-myc expression may be one pathway of apoptosis induction by vesnarinone in LU65A cells，and that vesnarinone may be useful to treat for lung cancer., 医歯学総合研究科博士論文(医学) ; 学位取得日: 平成12年4月21日},
 title = {ベスナリノン(vesnarinone)のヒト大細胞肺癌細胞株に対する増殖抑制およびアポトーシス誘導作用に関する研究},
 year = {2000},
 yomi = {ヤマグチ, アキヒコ}
}