{"created":"2023-07-25T08:04:16.355908+00:00","id":2439,"links":{},"metadata":{"_buckets":{"deposit":"baa3d975-58dd-4973-8c89-22d59ab3be18"},"_deposit":{"created_by":3,"id":"2439","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2439"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00002439","sets":["228:271:4976"]},"author_link":["121051","121049","121058","121055","11617","121054","8192","121053","121052","121061","121050","121059","121056","121057","121060"],"item_7_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Antimicrobial resistance of enteric bacteria isolated from bottlenose dolphin (Tursiops truncatus)"}]},"item_7_alternative_title_19":{"attribute_name":"タイトルよみ","attribute_value_mlt":[{"subitem_alternative_title":"ハンドウイルカ(Tursiops truncatus) ノ　チョウナイ　サイキン　ト　ソノ　ヤクザイ　タイセイ　ジョウキョウ"}]},"item_7_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicPageEnd":"72","bibliographicPageStart":"65","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"鹿兒島大學農學部學術報告=Bulletin of the Faculty of Agriculture, Kagoshima University"}]}]},"item_7_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-03-31"}]},"item_7_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"鯨類の腸内保有細菌は環境のモニタリング評価に有用と期待されるが, それらの分離報告は少ない｡かごしま水族館のハンドウイルカは錦江湾とつながった屋外水路での展示を通し, 湾内の細菌を摂取していると考えられ, 外部環境の影響を受けている可能性がある｡そこで, 直腸便から菌を分離同定し, E. coliとVibrio　科細菌について, パルスフィールドゲル電気泳動(PFGE)による遺伝子解析, 薬剤感受性試験を実施し, 薬剤耐性遺伝子の検索を行い錦江湾の影響を調査した｡2008年11月から2009年３月に得た14検体のうち, E.coli, Vibrio科細菌は, 各々９(64.3％), ６(42.9％)検体から分離され, 非病原性常在菌と考えられた｡PFGE解析の結果, E. coliは６クラスターに分類され, 各個体に固有の株の定着が確認された｡一方Vibrio科細菌では大きく５クラスターに分類され, 複数個体で同じクラスターの株を共有しており, 摂取後, 腸管内を通過していくと考えられた｡MIC測定の結果, Vibrio科細菌全����株はオキシテトラサイクリン(OTC), オフロキサシン(OFLX)に感受性だった｡E. coliは投薬歴がないOTCに７/45株(15.6％)が耐性で, tetA遺伝子のみを保有し, すべて水路に出る個体由来だったが, 投薬歴があるOFLXには25/34株(73.5％)が耐性で, 水路展示の有無とは無関係であり, 水族館外部からOTC耐性株が獲得されていると推測できる｡","subitem_description_type":"Other"},{"subitem_description":"Bacteria inhabiting the intestines of cetaceans are expected to be invaluable for environmental estimation. Some of the bottlenose dolphins (Tursiops truncates) kept at Kagoshima Aquarium are not only housed in indoors but are also able to use an outside waterway in Kinko-wan Bay. To determine the influence of the bay’s environment, intestinal bacteria were isolated from the cetaceans. Antimicrobial susceptibility and molecular characterization of the isolates were also carried out. Nine (64.3%) and six (42.6%) rectum samples collected between November 2008 and March 2009 were positive for Escherichia coli and Vibrionaceae, respectively. The isolated bacteria were considered to be non-pathogenic. PFGE analysis classified the E. coli isolates into 6 clusters. Each dolphin was considered to have its own type of E. coli. Whereas the isolates of Vibrionaceae were classified into 5 clusters, and the same types of isolates were common in different dolphins, suggesting that these bacteria would pass through the dolphins’ intestines. All 34 isolates of Vibrionaceae were sensitive to both oxytetracycline and ofloxacin. Seven E. coli isolates out of 45 carried the tetA gene and were resistant to OTC that had not been used for treatment. All resistant isolates were detected from the dolphins that sometimes used the outside waterway. Twenty-five of the 34 Vibrionaceae isolates were resistant to OFLX that had been used for treatment. There was no relation between the resistance to OFLX and whether the dolphins had been into the waterway. It may be inferred that OTC resistant bacteria isolates were acquired from outside the aquarium.","subitem_description_type":"Other"}]},"item_7_full_name_2":{"attribute_name":"著者よみ","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"キジマ, ショウコ"}]},{"nameIdentifiers":[{}],"names":[{"name":"チュウマ, タケヒサ"}]},{"nameIdentifiers":[{}],"names":[{"name":"オオツカ, ミカ"}]},{"nameIdentifiers":[{}],"names":[{"name":"ササキ, キョウコ"}]},{"nameIdentifiers":[{}],"names":[{"name":"オカモト, カロク"}]}]},"item_7_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"KIJIMA, Shoko"}]},{"nameIdentifiers":[{}],"names":[{"name":"CHUMA, Takehisa"}]},{"nameIdentifiers":[{}],"names":[{"name":"OTSUKA, Mika"}]},{"nameIdentifiers":[{}],"names":[{"name":"SASAKI, Kyoko"}]},{"nameIdentifiers":[{}],"names":[{"name":"OKAMOTO, Karoku"}]}]},"item_7_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"04530845","subitem_source_identifier_type":"ISSN"}]},"item_7_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00040603","subitem_source_identifier_type":"NCID"}]},"item_7_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"489","subitem_subject_scheme":"NDC"}]},"item_7_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_7_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_7_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"木嶋, 祥子"}],"nameIdentifiers":[{"nameIdentifier":"121049","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中馬, 猛久"}],"nameIdentifiers":[{"nameIdentifier":"11617","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000090201631","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]},{"creatorNames":[{"creatorName":"大塚, 美加"}],"nameIdentifiers":[{"nameIdentifier":"121050","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐々木, 恭子"}],"nameIdentifiers":[{"nameIdentifier":"121051","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡本, 嘉六"}],"nameIdentifiers":[{"nameIdentifier":"8192","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000000136847","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-27"}],"displaytype":"detail","filename":"AN00040603_v62_p65-72.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00040603_v62_p65-72.pdf","url":"https://ir.kagoshima-u.ac.jp/record/2439/files/AN00040603_v62_p65-72.pdf"},"version_id":"88aac50f-b06c-4ae2-8e37-0e0bacd3b3e8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"イルカ","subitem_subject_scheme":"Other"},{"subitem_subject":"大腸菌,","subitem_subject_scheme":"Other"},{"subitem_subject":"ビブリオ","subitem_subject_scheme":"Other"},{"subitem_subject":"薬剤耐性","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ハンドウイルカ(Tursiops truncatus) の腸内細菌とその薬剤耐性状況","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ハンドウイルカ(Tursiops truncatus) の腸内細菌とその薬剤耐性状況"}]},"item_type_id":"7","owner":"3","path":["44","4976"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-12-25"},"publish_date":"2014-12-25","publish_status":"0","recid":"2439","relation_version_is_last":true,"title":["ハンドウイルカ(Tursiops truncatus) の腸内細菌とその薬剤耐性状況"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-01-30T05:20:11.167914+00:00"}