@article{oai:ir.kagoshima-u.ac.jp:00002455,
 author = {SAKATA, Taizo and KAWAZU, Tsuyoshi},
 journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University},
 month = {2016-10-27},
 note = {Marine and fish isolates belonging to Vibrio damsela exhibited three characteristics consisting of hemolysin, serum resistance, and SDS-decomposition.
Complete hemolysis by the cell suspension of V. damsela Yp-16 isolated from
diseased yellowtail was observed after 30, 45, 90, and more than 300 min incubation for tilapia, eel, yellowtail, and human erythrocytes, respectively.
Hemolysin released by V. damsela Yp-16 was inhibited by adding Mg^{++} ion
to the reaction mixture with human erythrocytes, but not with tilapia erythrocytes.
However, if the bacterial suspension or culture supernatant containing Mg^{++} ion
at a high concentration was dialyzed against 1% NaCl, hemolytic activity for
human erythrocytes was recovered substantially.
Hemolysin production in the bacterial cells was repressed by adding antibiotics such as neomycin, chloramphenicol, and polymyxin B to the bacterial suspension.
Partially purified hemolysin from the strain Yp-16 was inactivated completely after heat treatment at above 56°C for 30 min. Optical temperature of the hemolysin for tilapia erythrocytes was found at 42°C.},
 pages = {9--18},
 title = {Production of Hemolysin by a Fish Pathogenic Strain of Vibrio damsela},
 volume = {41},
 year = {}
}