{"created":"2023-07-25T08:04:19.405459+00:00","id":2496,"links":{},"metadata":{"_buckets":{"deposit":"495c0708-ca9c-4122-a675-e09738ed86a6"},"_deposit":{"created_by":3,"id":"2496","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2496"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00002496","sets":["31:160:161"]},"author_link":["112112","13181","112111"],"item_8_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Development of a general method for obtaining double KO swine cells which will be used as donors for production of cloned homozygous piglets"}]},"item_8_alternative_title_19":{"attribute_name":"タイトルよみ","attribute_value_mlt":[{"subitem_alternative_title":"ホモ KO ブタ コタイ サクセイ ノ タメ ノ double KO サイボウ ノ ヒョウジュンテキ シュトクホウ ノ カイハツ"}]},"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-05-22"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2009-2011年度科学研究費助成事業(科学研究費補助金(基盤研究(C)))研究成果報告書　課題番号：21580350　研究代表者：佐藤正宏 (鹿児島大学フロンティアサイエンス研究推進センター教授)","subitem_description_type":"Other"},{"subitem_description":"本研究ではどんなタイプの遺伝子でもその遺伝子発現をノックアウトできるブタ細胞における汎用性の高いdouble KO細胞を作成する方法を開発することを目指した。具体的には、ヘテロKO細胞targeting vector （TV）を再度導入し、薬剤濃度を数倍高めた条件で選別する方法（repeated delivery of TV, RDTV）を用い、特定の遺伝子の発現を抑えることに成功した。","subitem_description_type":"Other"},{"subitem_description":"This study aims to develop a general method for obtaining double\nKO swine cels in which the target gene expression is completely blocked. This was\nsuccessfully performed by selection of heterogenous KO cels under higher concentrations\nof selective drugs after repeated delivery of a targeting vector (RDTV). These resulting\ndouble KO cells will be used for somatic cell nuclear transfer-mediated production of\ncloned pigs.","subitem_description_type":"Other"}]},"item_8_full_name_2":{"attribute_name":"著者よみ","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"サトウ, マサヒロ"}]}]},"item_8_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"SATO, Masahiro"}]}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"467","subitem_subject_scheme":"NDC"}]},"item_8_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_8_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"21580350"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"佐藤, 正宏"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-27"}],"displaytype":"detail","filename":"21580350seika.pdf","filesize":[{"value":"239.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"21580350seika.pdf","url":"https://ir.kagoshima-u.ac.jp/record/2496/files/21580350seika.pdf"},"version_id":"0336bac5-559a-49ec-b662-76a193726a73"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"KOブタ","subitem_subject_scheme":"Other"},{"subitem_subject":"ホモ","subitem_subject_scheme":"Other"},{"subitem_subject":"gene targeting","subitem_subject_scheme":"Other"},{"subitem_subject":"薬剤耐性","subitem_subject_scheme":"Other"},{"subitem_subject":"体細胞核移植","subitem_subject_scheme":"Other"},{"subitem_subject":"胎仔性繊維芽細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子導入","subitem_subject_scheme":"Other"},{"subitem_subject":"double KO","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ホモKOブタ個体作成のためのdouble KO細胞の標準的取得法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ホモKOブタ個体作成のためのdouble KO細胞の標準的取得法の開発"}]},"item_type_id":"8","owner":"3","path":["161"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-12-24"},"publish_date":"2014-12-24","publish_status":"0","recid":"2496","relation_version_is_last":true,"title":["ホモKOブタ個体作成のためのdouble KO細胞の標準的取得法の開発"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-09-01T10:38:36.559855+00:00"}