@article{oai:ir.kagoshima-u.ac.jp:00002571, author = {SATOH, Hikaru and CHING'ANG'A, H. M. and ILAILA, D. and KATAYAMA, Tadao C.}, journal = {南太平洋海域調査研究報告=Occasional papers}, month = {2016-10-27}, note = {During the trip from June 30 to August 10 in 1988, 105 seed samples of cultivated rice, Oryza sativa L., and a seed sample of O.glaberrima STEUD. were collected in Tanzania. Those were classified into 127 strains in O.sativa and 2 strains in O.glaberrima, according to the morphological observations. Seed storage proteins extracted from one rice seed of each strain were subjected to SDS-PAGE. The SDS-PAGE analysis of seed storage proteins was reported. Basing on the migration mode or staining intensity of the individual polypeptyde bands, strains were classified into three types for glutelin α subunits, tentatively named as types A, B and C (Fig. 2), and six types for 13 kDa polypeptides, tentatively named as types 1, 2, 3, 4, 5 and 6 (Fig. 3). Of 127 strains of O.sativa cultivars, 31 strains (24%) belonged to type A and 96 strains (76%) belonged to type B (Table 1). Type C was observed in two strains of O.glaberrima collected in Zanzibar Island. Geographical distribution of types A and B considerably differed among localities (Table 2). The frequencies of strains belonging to types A and B were 23% and 77% in Southwestern Area, 11% and 89% in Central and Western Areas, 33% and 67% in Northern Area, 16% and 84% in Eastern Area and 31% and 69% in Zanzibar and Pemba Islands, respectively. For 13 kDa polypeptides analysed by SDS-PAGE, types 1, 2, 3, 4 and 5 were found in the strains of O.sativa cultivars. Type 6 was observed in two strains of O.glaberrima (Table 1). The frequencies of strains belonging to types 1, 2, 3, 4 and 5 were found to be 20% (25 strains), 34% (43 strains), 24% (31 strains), 16.5% (21 strains), and 5.5% (7 strains), respectively. Geographical distribution of strains belonging to each type also differed considerably among the localities (Figs. 4A to 4E). The frequencies of strains belonging to types 1, 2, 3, 4 and 5 were found to be 23%, 54%, 11.5%, 11.5% and 0% in Southwestern Area, 53%, 26%, 11%, 5% and 5% in Central and Western Areas, 6%, 33%, 22%, 17% and 22% in Northern Area, 12%, 47%, 35%, 18% and 0% in Eastern Area and 13%, 22%, 36%, 24% and 4% in Zanzibar and Pemba Islands, respectively.}, pages = {114--126}, title = {SDS-PAGE Analysis of Storage Proteins of Cultivated Rice Collected in Tanzania, 1988}, volume = {18}, year = {} }