@article{oai:ir.kagoshima-u.ac.jp:00003796,
 author = {Hasui, Kazuhisa and Sato, Eiichi and Sueyoshi, Kazunobu and Kitajima, Shinichi and Goto, Masamitsu and Nakamura, Takao and Matsumoto, Daiten},
 journal = {Dendritic cells},
 month = {Jan},
 note = {In order to detect histochemically signals of human T-cell leukemia virus type 1 (HTLV-1) proviral DNA and its proviral DNA in MT-2 cells, we made highly biotinylated  probes corresponding to parts of HTLV-1 proviral DNA by means of 2 times polymerase chain reaction (PCR) and improved the procedures of DNA-RNA and DNA DNA cold in-situ-hybridization (cISH) for HTLV-1. We produced 7 highly biotinylated double-stranded DNA probes for the long terminal repeat, gag-pol region, env region  and pX tax and rex regions of HTLV-1. DNA-RNA cISH of these probes was established, evaluating the effect of 17% polyvinyl alcohol solvent for reducing non-specific alkaline phosphatase reaction in the process of visualizing hybridized probe and suggesting the necessary prehybridization incubation with hybridization buffer containing salmon DNA for diminishing non-specific binding of probes with tissue. On the other hand, DNA-DNA cISH of a cocktail of these 7 probes could show only too faint dot-like stain in nuclei to be applied to a study of HTLV-l-related lesions, denaturing DNA in sections and probes in aluminum box for 10 min at 200℃, incubating the sections with probes for hybridization for 3 days at 45℃ and performing overnight alkaline phosphatase reaction by using 17 % polyvinyl alcohol solvent after streptavidine-biotinylated alkaline phosphatase system to detect the hybridized probes.},
 pages = {27--35},
 title = {Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures},
 volume = {5},
 year = {1995}
}