{"created":"2023-07-25T08:05:22.369359+00:00","id":3796,"links":{},"metadata":{"_buckets":{"deposit":"f97498d6-606c-44cf-a4f3-f5ee4967be5f"},"_deposit":{"created_by":18,"id":"3796","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"3796"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00003796","sets":["57:78"]},"author_link":["116879","116880","116881","116882","116883","116884","116885"],"item_6_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"35","bibliographicPageStart":"27","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"Dendritic cells","bibliographic_titleLang":"en"}]}]},"item_6_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"1995-01-01","subitem_date_issued_type":"Issued"}]},"item_6_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"In order to detect histochemically signals of human T-cell leukemia virus type 1 (HTLV-1) proviral DNA and its proviral DNA in MT-2 cells, we made highly biotinylated  probes corresponding to parts of HTLV-1 proviral DNA by means of 2 times polymerase chain reaction (PCR) and improved the procedures of DNA-RNA and DNA DNA cold in-situ-hybridization (cISH) for HTLV-1. We produced 7 highly biotinylated double-stranded DNA probes for the long terminal repeat, gag-pol region, env region  and pX tax and rex regions of HTLV-1. DNA-RNA cISH of these probes was established, evaluating the effect of 17% polyvinyl alcohol solvent for reducing non-specific alkaline phosphatase reaction in the process of visualizing hybridized probe and suggesting the necessary prehybridization incubation with hybridization buffer containing salmon DNA for diminishing non-specific binding of probes with tissue. On the other hand, DNA-DNA cISH of a cocktail of these 7 probes could show only too faint dot-like stain in nuclei to be applied to a study of HTLV-l-related lesions, denaturing DNA in sections and probes in aluminum box for 10 min at 200℃, incubating the sections with probes for hybridization for 3 days at 45℃ and performing overnight alkaline phosphatase reaction by using 17 % polyvinyl alcohol solvent after streptavidine-biotinylated alkaline phosphatase system to detect the hybridized probes.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_6_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"日本樹状細胞研究会","subitem_publisher_language":"ja"},{"subitem_publisher":"Japanese Dendritic Cells Society","subitem_publisher_language":"en"}]},"item_6_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11039557","subitem_source_identifier_type":"NCID"}]},"item_6_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_6_subject_20":{"attribute_name":"NIIsubject","attribute_value_mlt":[{"subitem_subject":"医学","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_6_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hasui, Kazuhisa","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Eiichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sueyoshi, Kazunobu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kitajima, Shinichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Goto, Masamitsu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Takao","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumoto, Daiten","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-27"}],"displaytype":"detail","filename":"DC(Hasui)1995.pdf","filesize":[{"value":"3.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"DC(Hasui)1995.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/3796/files/DC(Hasui)1995.pdf"},"version_id":"621619bf-1001-4bc0-85be-80b36f55cde2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"HTLV-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA-DNA cold in-situ-hybridization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA-RNA cold-in-situ-hybridization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MT-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"biotinylated double-stranded DNA probes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"polymerase chain reaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures","subitem_title_language":"en"}]},"item_type_id":"6","owner":"18","path":["78"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-04-13"},"publish_date":"2010-04-13","publish_status":"0","recid":"3796","relation_version_is_last":true,"title":["Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-02-07T06:19:06.209714+00:00"}