@article{oai:ir.kagoshima-u.ac.jp:00004769, author = {KANAZAWA, Akio and TESHIMA, Shin-ichi and IMAI, Koichi}, journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University}, month = {2016-10-28}, note = {The biosynthesis of fatty acids from acetate-l-^<14>C, linoleic acid-l-^<14>C, and linolenic acid-l-^<14>C was examined on Tilapia zillii. Also, the bioconversion of linoleic acid-l-^<14>C to highly unsaturated fatty acids (HUFA) was investigated on the puffer fish, Fugu rubripes. After injection of the radioactive precursors, the proportional radioactivity was measured on each fatty acid which was isolated by means of thin-layer chromatography on AgNO_3-Kieselgel G and preparative gas-liquid chromatography. T.zillii was found to synthesize palmitic acid (16:0), palmitoleic acid (16:1), stearic acid (18:0), and oleic acid (18:1ω9) from acetate but not HUFA such as eicosapentaenoic acid (20:5ω3) and docosahexaenoic acid (22:6ω3). Also, T.zillii converted linolenic acid to 20:5ω3 and 22:6ω3, but the fish slightly metabolized linoleic acid to ω6 series of HUFA. The puffer fish converted linoleic acid to eicosadienoic acid (20:2ω6) and arachidonic acid (20:4ω6). These results were discussed in relation to the essential fatty acid requirements for T.zillii and the puffer fish.}, pages = {313--318}, title = {Biosynthesis of Fatty Acids in Tilapia zillii and the Puffer Fish}, volume = {29}, year = {} }