@article{oai:ir.kagoshima-u.ac.jp:00004814, author = {日高, 富男 and 白浜, 豊宏}, journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University}, month = {2016-10-28}, note = {A temperate phage designated 07T-12P was induced from a lysogenic strain (07T-12L) isolated from Kagoshima Bay mud. The lysogenic strain is a monotrichous polarly flagellated marine Pseudomonas sp. The phage 07T-12P give turbid plaque with diameter in 2-3mm on the sensitive strain (07T-12S) in duble agar layer plate. The electron micrograph of the phage particles shows that the structure of them has hexagonal and approximately 60 mμ in diameter head, and very small tail. The special character of the phage is to be very sensitive to heat and chloroform. When phage 07T-12P and strain 07T-12S were mixed at a MOI of 0.01 in sea water broth, there was 85-90 per-cent adsorption within 20 minutes at 25°C. The One-step growth curve of this phage shows that the latent period is 60 minutes, the rise period 35 minutes, and the average burst size is calculated to be 25 phage particles per infected cell. The phage yield was much lower. The cells of 07T-12L induced spontaneously the phage 07T-12P at a ratio of 0.8 per-cent of them. The phage induction rate of 07T-12L cells increased as high as 70-75 per-cent when they were irradiated ultraviolet light or treated with mitomycin C. On the other hand, when the phage 07T-12P was infected to 07T-12S cells at a MOI of 10, a 10-16 per-cent of the cells resulted in the lysogenic response and the others, a 84-90 per-cent of them resulted in the litic response. In this paper, the authors revealed the characteristics at the phases in the lysogenic cycle of the marine temperate phage system.}, pages = {137--148}, title = {海底泥より分離したテンペレート・ファージ系の性状}, volume = {23}, year = {} }