{"created":"2023-07-25T08:06:18.027707+00:00","id":5019,"links":{},"metadata":{"_buckets":{"deposit":"ebcf3cdf-0fbf-473f-aff8-27e35bab480e"},"_deposit":{"created_by":3,"id":"5019","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5019"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00005019","sets":["228:271:4976"]},"author_link":["98289","98291","98290","98292"],"item_7_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Aloe vera (L.) Burm. f.の染色体分析におけるEMA法, 蛍光染色及びrDNAのFISHの適用"}]},"item_7_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicPageEnd":"89","bibliographicPageStart":"83","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"鹿兒島大學農學部學術報告=Bulletin of the Faculty of Agriculture, Kagoshima University"}]}]},"item_7_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-03-31"}]},"item_7_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Fluorescent banding patterns and the locations of the ribosomal RNA gene (rDNA) of Indian Aloe vera (L.) Burm. f. chromosomes were determined. Root tips were pretreated in 2 mM 8- hydroxyquinoline at 10 to 12℃ for 3h. The chromosome samples were prepared by the enzymatic maceration and air-drying method (EMA). The best preparation, with all chromosomes relatively extended and well spread without cytoplasm, was obtained following enzymatic condition of 0.67% Cellulase Onozuka RS, 0.5% Macerozyme R200 and 0.1% Pectolyase Y-23 for 25 to 35 min at 37°C. No chromomycin A3(CMA) positive or negative bands were detected. Five 4'-6-diamidino-2-phenylindole (DAPI) positive bands, located at centromeric regions in three small and two large chromosomes, were detected with actinomycin D treatment. The 18S-5.8S-25S rDNA sites were detected in telomeric regions of the short arm of one small chromosome and the long arm of two large chromosomes by fluorescence in situ hybridization (FISH).","subitem_description_type":"Other"},{"subitem_description":"Aloe vera (L.) Burm. f. (アロエ) 染色体の蛍光分染及び染色体上でのリボゾームRNA遺伝子(rDNA)\nの位置の決定を実施した｡根端は2mMの8-hydroxyquinolineで10～12℃, ３時間前処理した｡染色体標本は酵素解離空気乾燥法(EMA) によって作製した｡全染色体が適度に拡がり, 細胞質の無い最も良好な標本は, 以下の条件で得られた；0.67％のCellulase Onozuka RS, 0.5％のMacerozyme R200及び.0.1％ Pectolyase Y-23で25～35分, 37℃｡Chromomycin A3(CMA) バンドは検出できなかった｡Actinomycin D処理を併用すると, 4'-6-diamidino-2-phenylindole (DAPI) ＋バンドが小染色体の動原部に３か所, 大染色体の端部に２か所の計５か所に出現した｡\n蛍光in situハイブリダイゼーション(FISH) によって, 18S-5.8S-25S rDNAは１本の小染色体の単腕端部\nに１か所, ２本の大染色体の長腕端部に各１か所位置することが明らかとなった｡","subitem_description_type":"Other"}]},"item_7_full_name_2":{"attribute_name":"著者よみ","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"ヤマモト, マサシ"}]}]},"item_7_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"山本, 雅"}]}]},"item_7_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"04530845","subitem_source_identifier_type":"ISSN"}]},"item_7_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00040603","subitem_source_identifier_type":"NCID"}]},"item_7_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"479","subitem_subject_scheme":"NDC"}]},"item_7_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_7_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_7_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"JHA, Timir Baran"}],"nameIdentifiers":[{"nameIdentifier":"98289","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"YAMAMOTO, Masashi"}],"nameIdentifiers":[{"nameIdentifier":"98290","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"AN00040603_v62_p83-89.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00040603_v62_p83-89.pdf","url":"https://ir.kagoshima-u.ac.jp/record/5019/files/AN00040603_v62_p83-89.pdf"},"version_id":"00432e06-826a-4750-a327-923733b50d52"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CMA","subitem_subject_scheme":"Other"},{"subitem_subject":"DAPI","subitem_subject_scheme":"Other"},{"subitem_subject":"fluorescence in situ hybridization","subitem_subject_scheme":"Other"},{"subitem_subject":"Indian Aloe","subitem_subject_scheme":"Other"},{"subitem_subject":"karyotype","subitem_subject_scheme":"Other"},{"subitem_subject":"蛍光in situハイブリダイゼーション","subitem_subject_scheme":"Other"},{"subitem_subject":"核型分析","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Application of EMA, Fluorescent Staining and FISH of rDNA in Analysis of Aloe vera (L.) Burm. f. Chromosomes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Application of EMA, Fluorescent Staining and FISH of rDNA in Analysis of Aloe vera (L.) Burm. f. Chromosomes"}]},"item_type_id":"7","owner":"3","path":["44","4976"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-12-25"},"publish_date":"2014-12-25","publish_status":"0","recid":"5019","relation_version_is_last":true,"title":["Application of EMA, Fluorescent Staining and FISH of rDNA in Analysis of Aloe vera (L.) Burm. f. Chromosomes"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-01-30T05:17:27.939007+00:00"}