{"created":"2023-07-25T08:06:19.983311+00:00","id":5058,"links":{},"metadata":{"_buckets":{"deposit":"230c2176-1b11-4399-b3ac-a7cbf4d047fe"},"_deposit":{"created_by":3,"id":"5058","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5058"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00005058","sets":["228:230:5162","57:58:59"]},"author_link":["119386","119388","119375","119385","119378","119376","119384","119377","119387","119374"],"item_7_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"イヌZUBECA4プライマーによって増幅されるニホンタヌキマイクロサテライト座について"}]},"item_7_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueNumber":"3","bibliographicPageEnd":"46","bibliographicPageStart":"41","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"鹿児島大学医学雑誌=Medical journal of Kagoshima University"}]}]},"item_7_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-01-01"}]},"item_7_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"The Japanese raccoon dog short tandem repeats (STRs, or microsatellites) were investigated using the primer set for the\nZUBECA4 locus, one of the dog STR primers. The properties of Japanese raccoon dog PCR products were examined by\nelectrophoresis and sequence analyses. Six alleles were detected by the electrophoresis analyses in nine Japanese raccoon\ndogs. The sequence analyses on the six alleles revealed that variants are based on the difference in the total number of\nrepeats of GAAA and GCAA. The repeating units of Japanese raccoon dogs were simpler than those of dog alleles. The\nsix alleles were designated as 12-16 and 18. Allele 15 and 18 were divided into 15^{12}, 15^{14}, 15^{15}, 18^{13} and 18^{14} sub-alleles by\nthe times GAAA was repeated. Their frequencies were estimated to be: allele 12=0.10, allele 13=0.17, allele 14=0.17, allele\n15^{12}=0.22, allele 15^{14}=0.06, allele 15^{15}=0.06, allele 16=0.06, allele 18^{13}=0.06 and allele 18^{14}=0.10. Heterozygosity was calculated\nto be 0.79 in STR alleles and 0.86 in sub-alleles. Further, new primers for Japanese raccoon dogs were developed based on\nthe sequences of the Japanese raccoon dog alleles. This primer set was successfully amplified as a small-sized amplicon,\nwhich is more suitable for practical uses than dog primers. These findings indicated that the ZUBECA4 locus is a quite useful STR for species identification, individual identification, pedigree study and population study on Japanese raccoon\ndogs.","subitem_description_type":"Other"}]},"item_7_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"林園, 典子"}]},{"nameIdentifiers":[{}],"names":[{"name":"吾郷, 一利"}]},{"nameIdentifiers":[{}],"names":[{"name":"林, 敬人"}]},{"nameIdentifiers":[{}],"names":[{"name":"吾郷, 美保子"}]},{"nameIdentifiers":[{}],"names":[{"name":"小片, 守"}]}]},"item_7_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03685063","subitem_source_identifier_type":"ISSN"}]},"item_7_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00040104","subitem_source_identifier_type":"NCID"}]},"item_7_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"493","subitem_subject_scheme":"NDC"}]},"item_7_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_7_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_7_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"HAYASHIZONO, Noriko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"AGO, Kazutoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HAYASHI, Takahito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"AGO, Mihoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OGATA, Mamoru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"AN00040104_v61n3_p41-46.pdf","filesize":[{"value":"246.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00040104_v61n3_p41-46.pdf","url":"https://ir.kagoshima-u.ac.jp/record/5058/files/AN00040104_v61n3_p41-46.pdf"},"version_id":"3fb18f52-dca3-4d65-b067-18d5ec820441"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"forensic genetics","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA polymorphism","subitem_subject_scheme":"Other"},{"subitem_subject":"STR","subitem_subject_scheme":"Other"},{"subitem_subject":"microsatellite","subitem_subject_scheme":"Other"},{"subitem_subject":"dog ZUBECA4 primers","subitem_subject_scheme":"Other"},{"subitem_subject":"Japanese raccoon dog","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Amplification of Microsatellites of Japanese Raccoon Dogs (Nyctereutes procyonoides viverrinus) with Primers for the Dog ZUBECA4 Locus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Amplification of Microsatellites of Japanese Raccoon Dogs (Nyctereutes procyonoides viverrinus) with Primers for the Dog ZUBECA4 Locus"}]},"item_type_id":"7","owner":"3","path":["59","5162"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-03"},"publish_date":"2012-07-03","publish_status":"0","recid":"5058","relation_version_is_last":true,"title":["Amplification of Microsatellites of Japanese Raccoon Dogs (Nyctereutes procyonoides viverrinus) with Primers for the Dog ZUBECA4 Locus"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-09-01T11:21:32.071875+00:00"}