@article{oai:ir.kagoshima-u.ac.jp:00005160,
 author = {PASCUAL, Felicitas P. and KANAZAWA, Akio},
 issue = {2},
 journal = {南海研紀要},
 month = {2016-10-28},
 note = {To determine the effects of deficiency of individual amino acids believed to be
essential to Penaeus monodon juveniles, a 28-day feeding experiment was carried out
with P.monodon postlarvae.
P.monodon postlarvae weighing around 120 mg each were randomly distributed in
individual perforated one liter jars, 10 jars in 60-liter, fiberglass tanks, in a flow through seawater system, which passed through a 0.35 micron filter before use. Animals were reared in these jars for 28 days on semi-purified moist diets. Salinity and temperature ranged from 27 to 32 ppt and 26 to 28.9°C, respectively.
Percentage weight gains and survival rates were not significantly different among
test diets. However, some amino acids seemed to be more critical than others in the
diets. Animals given phenylalanine-free, leucine-free, and methionine-free diets gained more than 300% compared to those fed the complete amino acid diets. A low weight
gain of 228.6% was observed in animals fed arginine-free diet. Animals fed the histidine-free, lysine-free, threonine-free and valine-free diets also gained less than 200%.
Survival rates were 60% for those given threonine-free and isoleucine-free diets, 70%
for histidine-free and complete amino acid diets, whereas those fed the phenylalanine-free, leucine-free and methionine-free diets had survival of 80 to 85%. These results suggest the possibility that some amino acids were taken from sources outside the
diets and could have come from whatever bacterial growth there was in the rearing jars.},
 pages = {65--72},
 title = {Specific Amino Acid-Free Semi-Purified Diets for Penaeus monodon Juveniles},
 volume = {7},
 year = {}
}