@article{oai:ir.kagoshima-u.ac.jp:00005236, author = {SATOH, Hikaru and RATOKONJANAHARY, Xavier Roland and KATAYAMA, Tadao C.}, journal = {南太平洋海域調査研究報告=Occasional papers}, month = {2016-10-28}, note = {During the trip from June 1 to June 28 in 1988, 99 seed samples of cultivated rice, Oryza sativa L., were collected. Those were classified into 130 strains according to the morphological observations. Seed storage proteins extracted from one rice seed of each strain were subjected to SDS-PAGE. The SDS-PAGE analysis of seed storage proteins was reported. Basing on the migration mode or staining intensity of the individual polypeptide bands, strains were classified into two types for glutelin α -3 subunit, tentatively named as types A and B (Fig. 2), and four types for 13 kDa polypeptides, tentatively named types 1, 2, 3 and 4 (Fig. 3). Of 130 strains, 21 strains (16% of the whole) belonged to type A and 109 strains (84%) belonged to type B (Table 1). The number of strains belonging to types A and B were 16 (14%) and 97 (86%) in the lowland rice, and 5 (29%) and 12 (71%) in the upland rice, respectively. Geographical distribution of types A and B considerably differed among localities (Table 2). The frequencies of strains belonging to types A and B were 14% and 86% in Northern Area, 7% and 93% in Central Area, 30% and 70% in Eastern Area and 12% and 88% in South Mountain Area, respectively. It seems that one of the reasons for the high frequencies of type A in Eastern Area is caused by the high frequency of type A in upland rices. The frequencies of strains belonging to types 1, 2, 3 and 4 were found to be 11% (14 strains), 30% (39 strains), 44% (57 strains) and 15% (20 strains), respectively. The frequencies of types 1, 2, 3 and 4 were found 9%, 30%, 45% and 16% in the lowland rice, and 24%, 29%, 35% and 12% in the upland rice, respectively. Geographical distribution of strains belonging to respective types also differed considerably among localities (Figs. 4A to 4D). The frequencies of strains belonging to types 1, 2, 3 and 4 were found to be 5%, 21%, 51% and 23% in Northern Area, 4%, 32%, 39% and 25% in Central Area, 21%, 21%, 52% and 6% in Eastern Area, and 15%, 54%, 27% and 4% in South Mountain Area, respectively.}, pages = {101--113}, title = {SDS-PAGE Analysis of Storage Proteins of Cultivated Rice Collected in Madagascar, 1988}, volume = {18}, year = {} }