@article{oai:ir.kagoshima-u.ac.jp:00005251,
 author = {Nishihara, Gregory N. and Mori, Yuko and 森, 裕子 and Terada, Ryuta and 寺田, 竜太 and Noro, Tadahide and 野呂, 忠秀},
 issue = {1},
 journal = {水産増殖},
 month = {},
 note = {A simplified method to isolate and cultivate the red alga, Laurencia brongniartii J. Agardh was established to prepare it for indoor tank cultivation. This method was addressed so that relatively inexperienced personnel can start and maintain laboratory cultures of the organism. The method uses the apical tissue of branch tips which are relatively free of epiphytes and requires almost no specialized equipment or facilities. The effect of size and location of apical tissue on the relative growth rates (%RGR) and survival rates (%SR) were examined to determine the size and location of apical tissue that would promote the highest survivability and fastest growth rates. The larger sizes (5.0 mm) showed higher %SR (62.5%) and smaller sizes (1.0 mm) showed higher %RGR (4.43%/day). There was also a significant effect of tissue location, with material closest to the apex showing the highest %RGR (2.16%/day). Different concentrations of nitrate were not significant, however higher concentrations appeared to have a negative impact on %RGR. Temperature and light had a significant effect on %RGR. Optimal growth was observed at 24 and 28°C and at an irradiance of 20μmol photons/m2/s., 紅藻ソゾノハナLaurencia brongniartii J. Agardh (イギス目) は, 含有する生理活性物質の重要性から, 有用海藻として資源の維持管理が試みられている。本研究では, 本種の養殖用母藻を室内で大量に培養するための簡易な単離法と培養法を確立し, 至適生育条件を検索した。最も良く生長する組織片を単離するため, 藻体の上端部から様々な長さや部位の組織片を摘出し, 生長率 (%RGR) と生存率 (%SR) を基に比較した。その結果, 大形の組織片 (5.0mm) には高い生存率 (62.5%) が見られ, 小形の組織片 (1.0mm) には高い生長率 (4.43%/day) が見られた。また, 組織片は摘出部位によって生長率に差が見られ, 先端に近い部位の生長率 (2.16%/day) が最も高かった。組織片の生長は水温と光量に強く制限され, 24℃および28℃で20μmol photons/m2/sが至適な条件だった。さらに, 組織片の生長は高濃度の硝酸態窒素濃度 (329.4μmol NO3-N/l) によっても制限された。本種の至適生育条件で単離培養法を用いると, 容易に養殖母藻を養成・供給できることが明らかになった。},
 pages = {1--10},
 title = {A Simplified Method to Isolate and Cultivate, Laurencia brongniartii J. Agardh (Rhodophyta, Ceramiales) from Kagoshima, Japan},
 volume = {52},
 year = {2004}
}