{"created":"2023-07-25T08:06:45.022186+00:00","id":5635,"links":{},"metadata":{"_buckets":{"deposit":"6f2de0ce-369d-4ca5-866a-f1f890f38047"},"_deposit":{"created_by":18,"id":"5635","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"5635"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00005635","sets":["57:80"]},"author_link":["28048"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-05-31","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2011-2012年度科学研究費助成事業(科学研究費補助金(若手研究(B))研究成果報告書　課題番号：23792240　研究代表者：葛西貴行(鹿児島大学・大学院医歯学総合研究科・助教)","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"骨芽細胞分化過程において、AMP-activated protein kinase(AMPK)のリン酸化レベルの低下が見られた。AMPK活性化剤metformin刺激により、基質石灰化の抑制，骨基質特異的タンパク質およびRunx2遺伝子発現レベルの低下，さらにALP活性の低下が認められた。活性型AMPK発現誘導によっても、基質石灰化が抑制された。骨折治療に応用されている低周波超音波（LIPUS）刺激を骨芽細胞に与えた結果、AMPKリン酸化レベルが低下した。LIPUSによるAMPKリン酸化の抑制が骨芽細胞分化を促進する可能性が考えられた。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"AMP-activated protein kinase(AMPK) is a kinase responds to mechanical stress. Phosphorylation of AMPKα was significantly decreased during osteoblastic differentiation in both primary osteoblasts and MC3T3-E1, a mouse osteoblastic cell line. Conversely, the terminal differentiation of primary osteoblasts and MC3T3-E1 cells, represented by matrix mineralization, was significantly inhibited by stimulation with metformin, an activator of AMPK. Matrix mineralization of MC3T3-E1 cells was also inhibited by the forced expression of a constitutively active form of AMPKα. Metformin significantly inhibited gene expression of Runx2 along with osteoblast differentiation markers. Low-intensity pulsed ultrasound (LIPUS), which is used clinically to promote bone fracture healing, decreased AMPKα phosphorylation. Thus, LIPUS may promote osteoblast differentiation through dephosphorylation of AMPK.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"497.5","subitem_subject_scheme":"NDC"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"23792240"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"葛西, 貴行","creatorNameLang":"ja"},{"creatorName":"KASAI, Takayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"28048","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000030457650","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"23792240.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"23792240.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/5635/files/23792240.pdf"},"version_id":"7a19a291-ea5e-47f3-bca5-4f0ea7f09087"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"AMPK","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"骨芽細胞","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"メカニカルストレス","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"シグナル伝達","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"LIPUS","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"骨芽細胞分の分化およびメカニカルストレス応答性におけるＡＭＰＫの役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"骨芽細胞分の分化およびメカニカルストレス応答性におけるＡＭＰＫの役割","subitem_title_language":"ja"},{"subitem_title":"Roles of AMPK in Osteogenic Differentiation","subitem_title_language":"en"}]},"item_type_id":"8","owner":"18","path":["80"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-10-01"},"publish_date":"2013-10-01","publish_status":"0","recid":"5635","relation_version_is_last":true,"title":["骨芽細胞分の分化およびメカニカルストレス応答性におけるＡＭＰＫの役割"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-05-24T00:24:06.410075+00:00"}