@phdthesis{oai:ir.kagoshima-u.ac.jp:00006851,
 author = {中務, 弘基 and Nakatsukasa, Hiroki},
 month = {2016-10-28, 2016-10-28, 2016-10-28},
 note = {学位論文の要旨, 博士論文全文, The symbiotic plasmid (pSym) of Rhizobium leguminosarum bv. trifolii 4S5, which carries Tn5-mob, was successfully transferred into Agrobacterium tumefaciens A136 by  using a conjugation method. The resulting transconjugants induced the development of ineffective nitrogen-fixing nodules on the roots of white clover seedlings. Depending on the manner in which the pSym was retained, the transconjugants were divided into two groups of strains, Afp and Afcs. pSym was retained as a plasmid in the Afp strains but was integrated into int gene encoding phage-related integrase on the linear chromosome of A. tumefaciens A136 in strain Afcs1 (one of the Afcs strains) to form a symbiosis island. Conjugation was performed between strain Afcs1 and R. leguminosarum bv. trifolii H1 (a pSym-cured derivative of wild-type strain 4S), and the Rhizobium H1tr strains were screened as transconjugants. Eighteen of the H1tr strains induced effective nitrogen-fixing nodules on the roots of the host plants. pSym was transferred into all of the transconjugants, except for strain H1tr1, at the same size as pSym of strain 4S5. In strain H1tr1, pSym was integrated into the chromosome as a symbiosis island. These data suggest that pSym can exist among Rhizobium and Agrobacterium strains both as a plasmid and as a symbiosis island with transposon mediation., 理工学研究科博士論文(理学) ; 学位取得日: 平成20年3月25日},
 school = {鹿児島大学},
 title = {AgrobacteriumとRhizobium間を移動する巨大プラスミドと共生能発現},
 year = {},
 yomi = {ナカツカサ, ヒロキ}
}