@misc{oai:ir.kagoshima-u.ac.jp:00000719,
 author = {榊, 正剛 and Sakaki, Seigo},
 month = {},
 note = {Heavy water in tissues may influence on the effects of boron neutron capture therapy (BNCT). If light water is replaced with heavy water in tissues， boron containing compounds and the other drugs might remain in the tumor tissues for a longer time. A neutron beam seems to penetrate deeper through the heavy water than through the light water in biological tissues. Therefore， the author had experimented on the rat C6 glioma with boron neutron capture therapy after the animals had been given heavy water in place of their normal water supply and furthermore experimentally the effect of heavy water has been investigated. 1. BNCT with heavy water. The investigation was carried out in Wistar albino rats weighing approximately 300g at the begining of the experiment. A thermal neutron fluence of 1.0x10^13 neutrons/cm^2 was planned. In the first experiment，1.0x10^5 cells were implanted stereotactically into the right caudate nucleus of four rats. Two weeks later，the author brought these rats to the Musashi Atomic Energy Research Laboratory. These rats were set on an acryl plate in the prone position after pentobarbital anesthesia. They were put into aluminum cans covered with a thin lead sheet to protect their bodies from the gamma ray contamination of the beam. The thickness of cans was 3mm. On the other hand，their restricted heads were exposed in the thermal neutron irradiation field. Two days after irradiation， these rats were sacrificed and coronal sections of brains were cut down. The specimens were stained with Hematoxylin-Eosin and examined histopathologically. In the second experiment，all rats were fed heavy water orally on demand for two days before irradiation and injected with 50μg/g-body weight boron compounds into the peritoneal cavity six hours before irradiation. The doses of thermal neutron in these two sequential experiments were determined by activation of gold foils set at the back of the rats' heads. The doses were 8.41x10^12 neutrons/cm^2 and 1.17x10^13 neutrons/cm^2 respectively，and were satisfactorily close to our initially anticipated fluence. The gamma ray dose was 156.1 rad on the body and 375.6 rad on the head of the animal. Histological findings in the first experiment were that three of four rats showed advanced tumor growth. On the other hand，only one rat in the second group showsd tumor growth and intratumoral necrosis.
2. Pharmacokinetic study on boron compound with heavy water. The investigation was carried out in Fisher rats weighing 150-200g at the begining of the experiment. 4.0x10^5 rat 9L gliosarcoma cells were implanted into the subcutaneous region of the right latus. 8 rats (group A) were injected with heavy water into the peritoneal cavity everyday for 5 days. 6 rats (group B) were injected with light water. On the 6th day, 100μg/g-body weight boron compounds (BSH) was injected into the peritoneal cavith of all rats. 4 rats of A group and 3 rats of B group were sacrified on 6 hours after boron injcction. Other rats were sacrified on 12 hours after boron injection. Boron concentration in brain tissue and blood and tumor tissue were calculated. Boron concentration in tumor tissue was higher in A group than in B group. Such an experimental results might be contributory to clinical application of boron neuton capture therapy of brain tumors in near future., 医歯学総合研究科博士論文(医学) ; 学位取得日: 平成7年3月14日},
 title = {ラット脳腫瘍モデルを用いた硼素中性子捕捉療法における重水の効用に関する研究},
 year = {1995},
 yomi = {サカキ, セイゴウ}
}