@article{oai:ir.kagoshima-u.ac.jp:00007414,
 author = {Shiragi, M. H. Kabir and Baque, M. A. and Nasiruddin, K. M.},
 issue = {1},
 journal = {南太平洋研究, South Pacific Study},
 month = {Jan},
 note = {An experiment on virus free in vitro banana plantlets production by the meristem cultured from virus infected four month old banana suckers cv. Amritasagar were used as explants. Four levels of BAP (0, 3, 4 and 5 mg/l), 5 levels of NAA (0.0, 1.0, 1.5, 2.0 and 2.5 mg/l) were used as treatment for shoot proliferation in experiment-I and Four levels of IBA (0, 1, 2 and 3 mg/l), 4 levels of NAA (0, 2, 3 and 4 mg/l) were used for root formation in experiment-II. ELISA test was conducted for the confirmation of virus eradication rate of regenerated plants and survival rate of regenerated plants in experiment-III. A significant variation was found among the treatments. Studies on the different concentrations of BAP + NAA on shoot proliferation and differentiation, reveals that 4 mg/l BAP + 1.5 mg/l NAA produced the greater number of shoots (4.58/explant), the longest shoot (3.56 cm) and the highest numbers of leaves 3.11/explant). On the contrary, plantlets were cultured on half strength of MS media in different levels of IBA and NAA.The highest number of vigorous roots (6.98/explant) and maximum root length (5 cm) were recorded from the treatment combination 2 mg/l IBA+2 mg/l NAA and 3 mg/l IBA+ 3 mg/l NAA, respectively. While less vigorous root was found in control treatments. The rate of virus free plant regeneration from banana bunchy top virus (BBTV) infected plants was 57.14% and from banana mosaic virus (BMV) infected sources was 64.28%. The highest plant survival rate 83.33% was recorded in a mixture of sand, soil and cow dung (1:1:1). On the other hand, 67.67% plant survival rate was found in a mixture of coir and soil (1:2). Eradication of banana viruses from infected plants by meristem cultured is a useful tactic that will be helpful for commercial exploitation and virus free plant production for use of farmer’s level.},
 pages = {17--41},
 title = {Eradication of Banana Bunchy Top Virus (BBTV) and Banana Mosaic Virus (BMV) from Infected Plant of Banana cv. Amritasagar Through Meristem Culture},
 volume = {29},
 year = {2008}
}