@article{oai:ir.kagoshima-u.ac.jp:00000745,
 author = {日高, 富男},
 journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University},
 month = {2016-10-27},
 note = {The author investigated the effects of cultural conditions, temperature and mineral
composition, on the propagation of five bacteriophage systems isolated from sea water. For test of them, a method of one-step growth experiment was used in this work. It is designed
to observe one cycle of phage propagation, i. e. adsorption, multiplication, and burst. The results were as follows.
First, the host bacteria grew within the range of temperature from 17° to 35°C with optimum at 32°C, however, the phages propagated only between 25° and 28°C with optimum at 25°C. Secondly, the host bacteria showed different behaviours on the mineral requirement for
growth. Tow of them required media supplemented 3% NaCl for growth, and the other three
required K-, Mg-, and Ca-salt as well as NaCl. But all of the tested phages propagated only in the same mineral condition as sea water. These results demonstrate that temperature and
mineral conditions for the phage propagation in host cell are more restrictive than that for the growth of host bacterium alone. The properties of the tested phages, psychrophilic and
specific mineral requirement for propagation, defined them as marine phages., 供試海洋ファージ系,06N-21P,06N-24P,06N-34P,06N-52P,06N-58Pの増殖に及ぼす培養温度と培地中無機塩組成の影響について検討した。その結果,各宿主菌の発育温度域は17~35°Cであり,発育至適温度は32°C付近にあった。これに対し,各供試ファージの菌体内増殖の温度域は宿主菌発育のそれに比して狭く25°~28°Cで,至適温度は宿主菌のそれより低い25°C前後にあった。また,各宿主菌の発育のための無機塩要求性には差異があり,それを型別すると,06N-21,06N-34はNaClを要求する好塩型,06N-24,06N-52,06N-58はNaCl,KCl,Mg-塩,Ca-塩などの海水無機塩成分を要求する海洋型であった。しかしそれらに感染するファージは,培地中の無機塩成分,NaCl,KCl,Mg-塩,Ca-塩が海水と同様な濃度組成にある時にのみ増殖した。このように宿主菌体内でのファージの増殖には,宿主菌が発育できる温度域や無機塩組成より狭く厳しい条件下においてのみ増殖することが知られた。そして供試ファージの増殖における低温性と,厳しい海水無機塩要求性は,それらが海洋ファージであることを性格づけていた。},
 pages = {133--146},
 title = {海洋バクテリオファージの増殖に及ぼす培養温度と培地中無機塩の影響},
 volume = {32},
 year = {}
}