@article{oai:ir.kagoshima-u.ac.jp:00007649,
 author = {上村, 芳三 and UEMURA, Yoshimitsu and 村山, 敬一 and MURAYAMA, Keiichi and 久保, 幹 and KUBO, Motoki and 甲斐, 建一 and KAI, Kenichi and 幡手, 泰雄 and HATATE, Yasuo},
 journal = {鹿児島大学工学部研究報告, The research reports of the Faculty of Engineering, Kagoshima University},
 month = {Nov},
 note = {A cultivation study of Bacillus subtilis MT-2/pMK1, which produced a highly thermostable neutral protease, was carried out to establish the production technology of the protease. The effect of medium composition on the growth and the protease production of the strain were investigated. Glucose was recommended as the carbon and energy sources for the strain, while, on the other hand, the metabolic intermediates might cause an enzyme repression. The dissolved oxygen in the broth might be one of the significant factors. Calcium did not show the significant effect on the growth and the protease production in the present case. It was suggested that the carbon source, amino acid, and vitamins and minerals were essential for the growth and the protease production.},
 pages = {81--85},
 title = {遺伝子組み替え枯草菌による耐熱性プロテアーゼ生産用培地に関する基礎的研究},
 volume = {31},
 year = {1989}
}