@article{oai:ir.kagoshima-u.ac.jp:00000766,
 author = {YAMASAKI, Shigehisa and 山崎, 繁久 and TANABE, Katsunori and 田辺, 克則 and HIRATA, Hachiro and 平田, 八郎},
 issue = {1},
 journal = {鹿児島大学水産学部紀要, Memoirs of Faculty of Fisheries Kagoshima University},
 month = {Dec},
 note = {Tests for food efficiency and characteristics of chilled and frozen Nannochloropsis sp. (marine
Chlorella) were conducted in order to know the applicability of preserved food in massculture of
rotifer, Brachionus plicatilis, in consideration of the maintenance of the water quality. Chilled alga
was prepared through concentration and preservation by chilling at 3°C. While frozen food was
prepared by freezing of the concentrated alga at -25°C within the period of 20 minutes. Both
types of food were preserved at the respective temperature for a month before the use in the
experiment.
As to food efficiency of preserved algae, no significant difference was observed on the population
growth of rotifers fed on both fresh and preserved algae. As to activity of preserved alga, chilled
alga represented the same growth ability of fresh one under high light intensity. However, at low
light intensity of 150 lx, growth was not observed. Frozen alga showed 3 times higher exudation of
organic nitrogen from cell, in comparison with chilled alga.},
 pages = {77--82},
 title = {Efficiency of Chilled and Frozen Nannochloropsis sp. (Marine Chlorella) for Culture of Rotifer},
 volume = {38},
 year = {1989}
}