{"created":"2023-07-25T08:08:19.865119+00:00","id":7682,"links":{},"metadata":{"_buckets":{"deposit":"8063d09a-9bd3-41e6-b1f0-a08d9df14369"},"_deposit":{"created_by":18,"id":"7682","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"7682"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00007682","sets":["82:83"]},"author_link":["29280"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2011-04-26","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2008-2010年度科学研究費補助金(基盤研究(C))研究成果報告書 課題番号：20590149　研究代表者：武田泰生 (鹿児島大学医学部・歯学部附属病院准教授)","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"跳躍伝導のKチャネルの局在に関与するCaspr2の細胞内部分と直接結合する分子として小胞体への蛋白質パッケージングに関わるcarboxypeptidase E(CPE)を同定した。これら両者間の相互作用を検討した結果、Caspr2とCPEはともにラット大脳皮質2, 3層のapical dendritesに共存し、細胞内のゴルジ/小胞体に共局在しており、Caspr2の膜輸送はCPEを介したtrans-Golgi networkによることを明らかにした。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"To investigate the molecular mechanism for trafficking of Contactin-associated protein (Caspr) 2 to the cem membrane, we identified carboxypeptidase E (CPE) as a binding partner of Caspr2. Both Caspr2- and CPE-like immunoreactivities (IR) were found to co-localize in the apical dendrites and cell bodies of rat cortical neurons. In subcellular localization analysis, Caspr2- and CPE-like IRs were comigrated in the fractions of Golgi/ER. When Caspr2 cDNA was transfected into COS-7 cells with or without CPE cDNA, Caspr2 localized only in Golgi/ER without CPE, but localized in both Golgi/ER and plasma membrane with CPE. Our data suggest that CPE may be a key molecule to regulate Caspr2 trafficking to the cell membrane.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"20590149"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"武田, 泰生","creatorNameLang":"ja"},{"creatorName":"TAKEDA, Yasuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"29280","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000060245462","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"20590149seika.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"20590149seika.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/7682/files/20590149seika.pdf"},"version_id":"a585885b-f069-4cdf-9037-1f919347ff51"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"臨床薬学","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"難治性てんかん","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"コンタクチン関連蛋白質","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"トランスゴルジ輸送系","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"難治性てんかんに関わるコンタクチン関連蛋白質の分子間相互作用の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"難治性てんかんに関わるコンタクチン関連蛋白質の分子間相互作用の解析","subitem_title_language":"ja"},{"subitem_title":"Analysis for molecular mechanism of contactin associated protein on intractable epilepsy","subitem_title_language":"en"}]},"item_type_id":"8","owner":"18","path":["83"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-01-06"},"publish_date":"2015-01-06","publish_status":"0","recid":"7682","relation_version_is_last":true,"title":["難治性てんかんに関わるコンタクチン関連蛋白質の分子間相互作用の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-06-10T05:23:15.377183+00:00"}