@article{oai:ir.kagoshima-u.ac.jp:00000799,
 author = {SAKATA, Taizo and YOSHIKAWA, Takeshi and NISHITARUMIZU, Satomi},
 journal = {鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University},
 month = {2016-10-27},
 note = {A marine bacterium, C55a-2 strain was selected among various algicidal bacteria isolated from coastal sea water of Kagoshima Bay,
Japan, which killed diatom cells on a double layer agar plate. The algicidal strain was identified as Pseudomonas sp. tentatively on
physiological and biochemical examinations.
Pseudomonas sp. C55a-2 was demonstrated to produce an algicidal substance extracellularly in marine nutrient medium Z-CII. An
algicidal substance was purified from the culture supernatant of strain C55a-2 by a series of purification steps including ethyl acetate
extraction, Sep-Pak treatment, Sephadex LH-20 column chromatography and high performance liquid chromatography (HPLC) with
Mightysil RP-18GP column. The substance was shown to have algicidal activity against Chaetoceros ceratosporum, Chattonella
marina and Heterosigma akashiwo strains by use of a microplate culture method.},
 pages = {17--25},
 title = {Purification of an Algicidal Substance Produced by Marine Pseudomonas sp. C55a-2},
 volume = {59},
 year = {}
}