{"created":"2023-07-25T08:08:42.594070+00:00","id":8151,"links":{},"metadata":{"_buckets":{"deposit":"6c97c8f0-a8c1-4f48-92f6-3578252b42e3"},"_deposit":{"created_by":18,"id":"8151","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8151"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00008151","sets":["57:80"]},"author_link":["39132"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-04-02","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2010-2011年度科学研究費助成事業(科学研究費補助金(若手研究(B)))研究成果報告書　課題番号：22791674　研究代表者：園田祥三 (鹿児島大学大学院医歯学総合研究科助教)","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"近年、Transwellを利用した網膜色素上皮細胞 (RPE)培養によって、細胞極性を示すRPE (polarized RPE) 培養が可能になっている。我々は、さらに培養法を改良し、ヒトおよびブタRPEで、細胞極性が高くより分化した培養法を確立した。極性RPEの特徴は、形態的には細胞表面に微絨毛がよく発達、tight junction, adherence junctionがよく発達し形態的に生体RPEときわめて類似。機能的には、trans epithelial resisatance (TER)値がブタで200Ω・cm2 以上)、permeability assayで自由な物質の透過が制御、さらにVEGFやPEDFの極性分泌が生体と同じであることを確認した。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"Our group attempted to establish a reproducible method for culturing highly polarized RPE and tried to elucidate the pathophysiological mechanism of retinal disease using polarized culture RPE.\nHighly polarized RPE cells were grown on Transwell^{TM} filters (12 mm, i.d). Primary cultures of RPE cells on T75 flask were seeded on fibronectin-coated Transwell^{TM}. RPE cells were cultured in 1% FBS for more than 4 weeks. As in native tissue, porcine RPE cells formed a monolayer, were well pigmented, and were arranged in a regular hexagonal array. The integrity of RPE layer was confirmed by expression of TJ proteins ZO-1 and occluding and well developed microvilli, localization of pigment on the apical side, nuclei on basal side, and presence of tight‐junctional complexes by electron microscopy. Well-differentiated RPE cells secreted VEGF-A preferentially to the basolateral side of the tissue. With respect to application of polarized culture RPE system, after stimulation with TNF-a, TER decreased about 15% of reduction in comparison to control at 24 hours, which was abolished by anti-TNF-a antibody. This reduction was inhibited by SB203580 inhibitor, but not by any other inhibitors.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"496","subitem_subject_scheme":"NDC"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"22791674"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"園田, 祥三","creatorNameLang":"ja"},{"creatorName":"SONODA, Shozo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"39132","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000020325806","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"22791674seika.pdf","filesize":[{"value":"307.5 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"22791674seika.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/8151/files/22791674seika.pdf"},"version_id":"581288d6-25f4-41ce-8bf3-c38c086b3d55"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"細胞・組織","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"polarized cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"網膜色素上皮","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"加齢黄斑変性","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"Transwellを利用した極性をもつ網膜色素上皮細胞の培養法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Transwellを利用した極性をもつ網膜色素上皮細胞の培養法の確立","subitem_title_language":"ja"},{"subitem_title":"Assay for establishment of highly polarized retinal pigment epithelial cells","subitem_title_language":"en"}]},"item_type_id":"8","owner":"18","path":["80"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-12-24"},"publish_date":"2014-12-24","publish_status":"0","recid":"8151","relation_version_is_last":true,"title":["Transwellを利用した極性をもつ網膜色素上皮細胞の培養法の確立"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-05-24T00:24:07.024084+00:00"}