@article{oai:ir.kagoshima-u.ac.jp:00008228,
 author = {KIHARA, Hiroshi and ANRAKU, Mitsuo and HASHIMURA, Saburo},
 journal = {南方海域調査研究報告=Occasional Papers},
 month = {2016-10-28},
 note = {The ethanolic extracts of the sea urchin, belonging to the family Diadematidae,
had a hemolytic activity and toxicity to killifish. The heart of bull frog perfused with the solution containing the extracts was arrested in systole. These seemed to be due to a saponin-like agent which was the common component of sea animals, sea stars and sea cucumbers. In the isolated bull frog nerve-muscle preparation, the extracts abolished nerve stimulated contractions but did not affect the contractions produced by
direct stimulations of the muscle. In addition, the extracts caused a significant increase in miniature endplate potentials (MEPPs) frequency at a neuromuscular junction and this effect was reversible. Both activities on the muscle contraction and on the MEPPs frequency were separated from the saponin-like agent by a dialysis or gelfiltrations on Sephadex G-10, G-25 and Bio Gel P-4. This factor (s) in the ethanolic extracts was partially purified.},
 pages = {209--218},
 title = {Some Biological and Physiological Properties of the Toxins from the Sea Urchin, Family Diadematidae},
 volume = {5},
 year = {}
}