@article{oai:ir.kagoshima-u.ac.jp:00008447,
 author = {SHIMIZU, Keiichi and HASHIMOTO, Fumio},
 journal = {鹿兒島大學農學部學術報告=Bulletin of the Faculty of Agriculture, Kagoshima University},
 month = {2016-10-28},
 note = {The new activation-tagging vectors pKANACB, pKANACK and pKANACH were constructed.
The constructs contain a tetramer of the cauliflower mosaic virus 35S enhancer sequence, a selection
marker gene for plant transformation, an ampicillin resistance gene and the replication origin of
Escherichia coli which can be used for plasmid rescue within the T-DNA. These vectors can be stably
maintained in Agrobacterium tumefaciens strains GV3101, LBA4404 and AGL0. The plasmid selection
in E. coli and A. tumefaciens was accomplished via an ampicillin resistance gene. When tomato cotyledons
were infected with A. tumefaciens strain AGLO harboring the activation-tagging vectors (AGL0
pKANACB, AGL0-pKANACK and AGL0-pKANACH), glufosinate-, kanamycin- and hygromycinresistant
plants, respectively, were generated. The sequences of the T-DNA regions of pKANACB,
pKANACK and pKANACH have been deposited in GenBank under accession numbers AB775897,
AB777654 and AB777653, respectively., 新規の植物のアクティベーションタギングベクターpKANACB,pKANACK及びpKANACHを構築した。これらのベクターはT-DNA領域中にエンハンサー配列,植物形質転換のマーカー遺伝子とプラスミドの構成要素配列を持っており,多くのアグロバクテリウム中で維持できる。バクテリアでの選抜にはアンピシリン耐性遺伝子が利用できる。アグロバクテリウムの系統AGL0に,これらのベクターを導入してトマト
の子葉に感染させると,pKANACBではグルホシネート耐性,pKANACKではカナマイシン耐性,pKANACHではハイグロマイシン耐性の植物体が再生した。これらベクター配列のGenBankにおけるアクセッション番号はpKANACB:AB775897,pKANACK:AB777654,pKANACH:AB777653である。},
 pages = {39--47},
 title = {A new series of activation-tagging vectors : pKANACB, pKANACK and pKANACH},
 volume = {63},
 year = {}
}