{"created":"2023-07-25T08:08:58.882223+00:00","id":8447,"links":{},"metadata":{"_buckets":{"deposit":"0f6b4469-03d2-4431-bff1-79164939d1b0"},"_deposit":{"created_by":3,"id":"8447","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"8447"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00008447","sets":["228:271:4977"]},"author_link":["98600","98601","98599","98598"],"item_7_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"新規アクティベーションタギングベクターpKANACB, pKANACK及びpKANACH"}]},"item_7_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicPageEnd":"47","bibliographicPageStart":"39","bibliographicVolumeNumber":"63","bibliographic_titles":[{"bibliographic_title":"鹿兒島大學農學部學術報告=Bulletin of the Faculty of Agriculture, Kagoshima University"}]}]},"item_7_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-03-29"}]},"item_7_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"The new activation-tagging vectors pKANACB, pKANACK and pKANACH were constructed.\nThe constructs contain a tetramer of the cauliflower mosaic virus 35S enhancer sequence, a selection\nmarker gene for plant transformation, an ampicillin resistance gene and the replication origin of\nEscherichia coli which can be used for plasmid rescue within the T-DNA. These vectors can be stably\nmaintained in Agrobacterium tumefaciens strains GV3101, LBA4404 and AGL0. The plasmid selection\nin E. coli and A. tumefaciens was accomplished via an ampicillin resistance gene. When tomato cotyledons\nwere infected with A. tumefaciens strain AGLO harboring the activation-tagging vectors (AGL0\npKANACB, AGL0-pKANACK and AGL0-pKANACH), glufosinate-, kanamycin- and hygromycinresistant\nplants, respectively, were generated. The sequences of the T-DNA regions of pKANACB,\npKANACK and pKANACH have been deposited in GenBank under accession numbers AB775897,\nAB777654 and AB777653, respectively.","subitem_description_type":"Other"},{"subitem_description":"新規の植物のアクティベーションタギングベクターpKANACB,pKANACK及びpKANACHを構築した。これらのベクターはT-DNA領域中にエンハンサー配列,植物形質転換のマーカー遺伝子とプラスミドの構成要素配列を持っており,多くのアグロバクテリウム中で維持できる。バクテリアでの選抜にはアンピシリン耐性遺伝子が利用できる。アグロバクテリウムの系統AGL0に,これらのベクターを導入してトマト\nの子葉に感染させると,pKANACBではグルホシネート耐性,pKANACKではカナマイシン耐性,pKANACHではハイグロマイシン耐性の植物体が再生した。これらベクター配列のGenBankにおけるアクセッション番号はpKANACB:AB775897,pKANACK:AB777654,pKANACH:AB777653である。","subitem_description_type":"Other"}]},"item_7_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"清水, 圭一"}]},{"nameIdentifiers":[{}],"names":[{"name":"橋本, 文雄"}]}]},"item_7_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"04530845","subitem_source_identifier_type":"ISSN"}]},"item_7_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00040603","subitem_source_identifier_type":"NCID"}]},"item_7_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"467","subitem_subject_scheme":"NDC"}]},"item_7_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_7_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_7_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SHIMIZU, Keiichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HASHIMOTO, Fumio"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"AN00040603_v63_p39-47.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00040603_v63_p39-47.pdf","url":"https://ir.kagoshima-u.ac.jp/record/8447/files/AN00040603_v63_p39-47.pdf"},"version_id":"1beac016-086f-44f9-acf2-450c45a62740"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"activation tagging","subitem_subject_scheme":"Other"},{"subitem_subject":"functional genomics","subitem_subject_scheme":"Other"},{"subitem_subject":"mutation","subitem_subject_scheme":"Other"},{"subitem_subject":"T-DNA","subitem_subject_scheme":"Other"},{"subitem_subject":"transgenic plant","subitem_subject_scheme":"Other"},{"subitem_subject":"アクティベーションタギング","subitem_subject_scheme":"Other"},{"subitem_subject":"機能ゲノム科学","subitem_subject_scheme":"Other"},{"subitem_subject":"突然変異","subitem_subject_scheme":"Other"},{"subitem_subject":"形質転換植物","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A new series of activation-tagging vectors : pKANACB, pKANACK and pKANACH","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A new series of activation-tagging vectors : pKANACB, pKANACK and pKANACH"}]},"item_type_id":"7","owner":"3","path":["44","4977"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-05-21"},"publish_date":"2013-05-21","publish_status":"0","recid":"8447","relation_version_is_last":true,"title":["A new series of activation-tagging vectors : pKANACB, pKANACK and pKANACH"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-01-30T05:16:17.371816+00:00"}