@article{oai:ir.kagoshima-u.ac.jp:00008592, author = {Yamamoto, Masashi and 山本, 雅史 and Terakami, Shingo and 寺上, 伸吾 and Yamamoto, Toshiya and 山本, 俊哉 and Takada, Norio and 高田, 教臣 and Kubo, Tatsuya and 久保, 達也 and Tominaga, Shigeto and 冨永, 茂人}, issue = {4}, journal = {Journal of the Japanese Society for Horticultural Science}, month = {}, note = {The locations of the 18S-5.8S-25S ribosomal RNA gene (rDNA) in the seedlings of Japanese pear [Pyrus pyrifolia (Burm. F.) Nakai] ‘Osa Gold’ and European pear (P. communis L.) ‘La France’ and the 18S ribosomal rDNA in ‘Osa Gold’ were determined using fluorescence in situ hybridization (FISH). 18S-5.8S-25S and 18S rDNA probes were labeled with biotin-16-dUTP. The probes were detected using a fluorescein isothiocyanate (FITC)-avidin conjugate with chromosomes counterstained with propidium iodide (PI). The 18S-5.8S-25S and 18S rDNA sites of ‘Osa Gold’ and the former of ‘La France’ were detected in telomeric positions of six chromosomes. The number and positions of rDNA sites were stable.}, pages = {335--339}, title = {Detection of the ribosomal RNA gene in pear (Pyrus spp.) using fluorescence in situ hybridization}, volume = {79}, year = {2010}, yomi = {ヤマモト, マサシ and テラカミ, シンゴ and ヤマモト, トシヤ and タカダ, ノリオ and クボ, タツヤ and トミナガ, シゲト} }