@article{oai:ir.kagoshima-u.ac.jp:00008596,
 author = {Yamamoto, Masashi and 山本, 雅史 and Takada, Norio and 高田, 教臣 and Yamamoto, Toshiya and 山本, 俊哉 and Terakami, Shingo and 寺上, 伸吾 and Shigeta, Narumi and 滋田, 徳美 and Kubo, Tatsuya and 久保, 達也 and Tominaga, Shigeto and 冨永, 茂人},
 issue = {1},
 journal = {Journal of the Japanese Society for Horticultural Science},
 month = {},
 note = {Fluorescent banding patterns of pear chromosomes were determined from samples taken from root tips of open-pollinated seedlings of Pyrus pyrifolia (Burm. F) Nakai (Japanese pear), P. calleryana Decne. (Callery pear), P. pyrifolia × P. ussuriensis var. aromatica (a hybrid of Japanese pear and Iwateyamanashi), and P. mikawana Koidz. (Toyotomi Nashi). All accessions used in this study had 2n = 34 chromosomes. Chromomycin A3 (CMA)-positive (+) bands were observed in telomeric positions of four chromosomes in all accessions. 4'-6-diamidino-2-phenylindole (DAPI)-negative bands (−) corresponded with CMA+ bands. Fluorescence in situ hybridization (FISH) was conducted using open-pollinated seedlings of ‘Osa Gold’ (Pyrus pyrifolia) and Toyotomi Nashi (P. mikawana) as materials. Four out of six 18S-5.8S-25S ribosomal RNA gene (rDNA) sites corresponded with CMA+/DAPI− bands. The 5S rDNA sites were detected in centromeric positions of two chromosomes. Two centromeric 5S rDNA and six telomeric 18S-5.8S-25S rDNA sites were located on different chromosomes as determined from the results of multi-color FISH., ニホンナシ‘埼玉8’［Pyrus pyrifolia（Burm. F.）］，マメナシ‘愛知マメナシ’（P. calleryana Decne.），ニホンナシとイワテヤマナシとの交雑種‘岩手7号’（P. pyrifolia × P. ussuriensis var. aromatica）およびトヨトミナシ（P. mikawana Koidz.）の自然交雑実生を用いて，染色体の蛍光染色を実施した．供試全系統の染色体数は 2n = 34 であった．クロモマイシン A3（CMA）染色を行うと，全系統で4本の染色体の端部に CMA+ バンドが確認できた．4'-6-ディアミディノ-2-フェニルインドール二塩酸塩 (DAPI)− バンドの位置は CMA+ バンドと一致した．続いて，蛍光 in situ ハイブリダイゼーション（FISH）を‘おさゴールド’（Pyrus pyrifolia）およびトヨトミナシ自然交雑実生を用いて行った．6ケ所の 18S-5.8S-25S rDNA 遺伝子のうち，4ケ所は CMA+/DAPI− バンドの位置と一致した．5S rDNA 遺伝子は，2本の染色体の動原体近傍に確認できた．動原体近傍の2ケ所の 5S rDNA と端部の6ケ所の 18S-5.8S-25S rDNA 遺伝子は，各々別の染色体に存在した．},
 pages = {35--40},
 title = {Fluorescent Staining and Fluorescence in situ Hybridization of rDNA of Chromosomes in Pear (Pyrus spp.)},
 volume = {81},
 year = {2012},
 yomi = {ヤマモト, マサシ and タカダ, ノリオ and ヤマモト, トシヤ and テラカミ, シンゴ and シゲタ, ナルミ and クボ, タツヤ and トミナガ, シゲト}
}