{"created":"2023-07-25T08:09:06.076259+00:00","id":8596,"links":{},"metadata":{"_buckets":{"deposit":"b66e7f17-4ed9-4d88-bc90-49c4ad1469df"},"_deposit":{"created_by":18,"id":"8596","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8596"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00008596","sets":["43:125"]},"author_link":["121886","121887","121888","121889","121890","121891","121892"],"item_6_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"40","bibliographicPageStart":"35","bibliographicVolumeNumber":"81","bibliographic_titles":[{"bibliographic_title":"Journal of the Japanese Society for Horticultural Science","bibliographic_titleLang":"en"}]}]},"item_6_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2012-01-01","subitem_date_issued_type":"Issued"}]},"item_6_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Fluorescent banding patterns of pear chromosomes were determined from samples taken from root tips of open-pollinated seedlings of Pyrus pyrifolia (Burm. F) Nakai (Japanese pear), P. calleryana Decne. (Callery pear), P. pyrifolia × P. ussuriensis var. aromatica (a hybrid of Japanese pear and Iwateyamanashi), and P. mikawana Koidz. (Toyotomi Nashi). All accessions used in this study had 2n = 34 chromosomes. Chromomycin A3 (CMA)-positive (+) bands were observed in telomeric positions of four chromosomes in all accessions. 4'-6-diamidino-2-phenylindole (DAPI)-negative bands (−) corresponded with CMA+ bands. Fluorescence in situ hybridization (FISH) was conducted using open-pollinated seedlings of ‘Osa Gold’ (Pyrus pyrifolia) and Toyotomi Nashi (P. mikawana) as materials. Four out of six 18S-5.8S-25S ribosomal RNA gene (rDNA) sites corresponded with CMA+/DAPI− bands. The 5S rDNA sites were detected in centromeric positions of two chromosomes. Two centromeric 5S rDNA and six telomeric 18S-5.8S-25S rDNA sites were located on different chromosomes as determined from the results of multi-color FISH.","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"ニホンナシ‘埼玉8’［Pyrus pyrifolia（Burm. F.）］，マメナシ‘愛知マメナシ’（P. calleryana Decne.），ニホンナシとイワテヤマナシとの交雑種‘岩手7号’（P. pyrifolia × P. ussuriensis var. aromatica）およびトヨトミナシ（P. mikawana Koidz.）の自然交雑実生を用いて，染色体の蛍光染色を実施した．供試全系統の染色体数は 2n = 34 であった．クロモマイシン A3（CMA）染色を行うと，全系統で4本の染色体の端部に CMA+ バンドが確認できた．4'-6-ディアミディノ-2-フェニルインドール二塩酸塩 (DAPI)− バンドの位置は CMA+ バンドと一致した．続いて，蛍光 in situ ハイブリダイゼーション（FISH）を‘おさゴールド’（Pyrus pyrifolia）およびトヨトミナシ自然交雑実生を用いて行った．6ケ所の 18S-5.8S-25S rDNA 遺伝子のうち，4ケ所は CMA+/DAPI− バンドの位置と一致した．5S rDNA 遺伝子は，2本の染色体の動原体近傍に確認できた．動原体近傍の2ケ所の 5S rDNA と端部の6ケ所の 18S-5.8S-25S rDNA 遺伝子は，各々別の染色体に存在した．","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"園芸学会","subitem_publisher_language":"ja"}]},"item_6_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2503/jjshs1.81.35","subitem_relation_type_select":"DOI"}}]},"item_6_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"園芸学会","subitem_rights_language":"ja"}]},"item_6_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18823351","subitem_source_identifier_type":"PISSN"}]},"item_6_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA12177046","subitem_source_identifier_type":"NCID"}]},"item_6_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"625","subitem_subject_scheme":"NDC"}]},"item_6_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamamoto, Masashi","creatorNameLang":"en"},{"creatorName":"ヤマモト, マサシ","creatorNameLang":"ja-Kana"},{"creatorName":"山本, 雅史","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takada, Norio","creatorNameLang":"en"},{"creatorName":"タカダ, ノリオ","creatorNameLang":"ja-Kana"},{"creatorName":"高田, 教臣","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Toshiya","creatorNameLang":"en"},{"creatorName":"ヤマモト, トシヤ","creatorNameLang":"ja-Kana"},{"creatorName":"山本, 俊哉","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Terakami, Shingo","creatorNameLang":"en"},{"creatorName":"テラカミ, シンゴ","creatorNameLang":"ja-Kana"},{"creatorName":"寺上, 伸吾","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shigeta, Narumi","creatorNameLang":"en"},{"creatorName":"シゲタ, ナルミ","creatorNameLang":"ja-Kana"},{"creatorName":"滋田, 徳美","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kubo, Tatsuya","creatorNameLang":"en"},{"creatorName":"クボ, タツヤ","creatorNameLang":"ja-Kana"},{"creatorName":"久保, 達也","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tominaga, Shigeto","creatorNameLang":"en"},{"creatorName":"トミナガ, シゲト","creatorNameLang":"ja-Kana"},{"creatorName":"冨永, 茂人","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"YAMAMOTO_J-Japan-Soc-Hort-Sci_v81n1.pdf","filesize":[{"value":"470.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"YAMAMOTO_J-Japan-Soc-Hort-Sci_v81n1.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/8596/files/YAMAMOTO_J-Japan-Soc-Hort-Sci_v81n1.pdf"},"version_id":"e0474d0a-349e-480b-963e-2b28b451ccce"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CMA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DAPI","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FISH","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"McFISH","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ribosomal RNA gene","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Fluorescent Staining and Fluorescence in situ Hybridization of rDNA of Chromosomes in Pear (Pyrus spp.)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Fluorescent Staining and Fluorescence in situ Hybridization of rDNA of Chromosomes in Pear (Pyrus spp.)","subitem_title_language":"en"}]},"item_type_id":"6","owner":"18","path":["125"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-07-29"},"publish_date":"2013-07-29","publish_status":"0","recid":"8596","relation_version_is_last":true,"title":["Fluorescent Staining and Fluorescence in situ Hybridization of rDNA of Chromosomes in Pear (Pyrus spp.)"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-02-07T05:12:19.359162+00:00"}