{"created":"2023-07-25T08:09:08.654835+00:00","id":8653,"links":{},"metadata":{"_buckets":{"deposit":"01f898c8-e64f-4025-a057-5b113ad364ca"},"_deposit":{"created_by":18,"id":"8653","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8653"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00008653","sets":["57:80"]},"author_link":["42041"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-05-30","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2011-2012年度科学研究費助成事業(科学研究費補助金(若手研究(B))研究成果報告書　課題番号：23790251　研究代表者：蓑部悦子(鹿児島大学・大学院医歯学総合研究科・助教)","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"L型Caチャネルとその変異体の活性をinside-outパッチ法を用いて比較し、カルモジュリン（CaM）による調節について検討した。野生型チャネルは、CaM＋ATPまたはCaM＋オカダ酸で同程度の活性を示した。変異体チャネルでは、ATPがチャネルのslowな不活性化を抑制し、活性を維持するのに対し、オカダ酸による効果は認められなかった。このことからATPの作用はリン酸化に関連するものではないこと、オカダ酸は、変異体にはないチャネルC末端遠位部による作用を抑制することが示唆された。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"Properties of the L-type Ca2+ channel and its mutants were explored using inside-out patch-clamp technique. Activity of these channels was induced by calmodulin plus ATP. A protein phosphatase inhibitor okadaic acid could substitute for ATP in wild-type channels, but it not in the mutant channels. It was suggested that the effects of ATP and okadaic acid, although apparently similar, were mediated by different mechanisms.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"491.3","subitem_subject_scheme":"NDC"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"23790251"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"蓑部, 悦子","creatorNameLang":"ja"},{"creatorName":"MINOBE, Etsuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"42041","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000000448581","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"23790251.pdf","filesize":[{"value":"194.3 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"23790251.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/8653/files/23790251.pdf"},"version_id":"16b55dcf-30e1-4409-90cc-bbcdd846c977"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"カルシウムチャネル","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"カルモジュリン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"パッチクランプ法","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"inside-out","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"run-down","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"リン酸化","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"カルモジュリンをリンクさせた変異体を用いたＬ型Ｃａチャネルの活性調節機構の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"カルモジュリンをリンクさせた変異体を用いたＬ型Ｃａチャネルの活性調節機構の解析","subitem_title_language":"ja"},{"subitem_title":"Properties of the L-type Ca2+ channel and its mutants","subitem_title_language":"en"}]},"item_type_id":"8","owner":"18","path":["80"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-10-01"},"publish_date":"2013-10-01","publish_status":"0","recid":"8653","relation_version_is_last":true,"title":["カルモジュリンをリンクさせた変異体を用いたＬ型Ｃａチャネルの活性調節機構の解析"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-05-24T00:24:45.535574+00:00"}