{"created":"2023-07-25T08:09:08.746682+00:00","id":8655,"links":{},"metadata":{"_buckets":{"deposit":"2588d90f-7a7b-457e-8b27-eceafcd5e647"},"_deposit":{"created_by":18,"id":"8655","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8655"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00008655","sets":["82:83"]},"author_link":["42047"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-04-25","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2011-2012年度科学研究費助成事業(科学研究費補助金(若手研究(B))研究成果報告書　課題番号：23791995　研究代表者：山下高明(鹿児島大学・医学部・歯学部附属病院・助教)","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"硝子体内薬物投与に起因する炎症が網膜色素上皮に与える影響を調べる為に、炎症性サイトカインの代表であるTNF-αについて調査・報告した。当教室で培養可能な極性をもった網膜色素上皮細胞のシートにTNF-αを暴露させると、細胞同士のタイトジャンクション（ZO-1）の破壊が認められた。さらに、TNF-αによるさまざまな細胞内シグナル伝達の中で、MAPキナーゼパスウェイが活性化されることにより、タイトジャンクションの破壊、すなわち外側血液網膜関門の破壊が引き起こされることが分かった。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"Retinal pigment epithelial (RPE) cells form a blood-ocular barrier, and their polarized property is crucial for maintaining the barrier functions. Tumor necrosis factor alpha (TNF-α), a major pleotropic inflammatory cytokine that disrupts the barrier function and eventual angiogenesis, is expressed in the choroidal neovascularizations of age-related macular degeneration eyes. Thus, it most likely plays an important role in the progression of the disease. The purpose of this study was to compare the effects of TNF-α on the barrier function of polarized RPE cells. Non-polarized RPE cells were used as negative controls. Isolated porcine RPE cells were seeded on Transwell™ membranes. The polarization of the RPE cells was determined by their high transepithelial electrical resistance and by their differential secretion of vascular endothelial growth factor (lower layer/upper layer >2.5X). Polarized RPE cells were incubated with 10 ng/ml of TNF-α and the TER was measured. TNF-α significantly decreased the TER of polarized RPE cells by 17.6 ± 2.7% (P < 0.001) of the control at 24 h and that of non-polarized RPE cells by 5.4 ± 6.5% (P = 0.401). The p38 mitogen-activated protein kinase (MAPK) inhibitor, SB203580, blocked the effects of TNF-α of decreasing the TER. Cell junction-related molecules, e.g., ZO-1, located between cells in control RPE cells, were disassembled by TNF-α, and this breakdown was suppressed by SB203580 in polarized RPEs. These results indicate that the breakdown of the RPE barrier function was caused exclusively by TNF-α in polarized RPEs, and TNF-α was acting through the p38 MAPK pathways. Investigations of polarized RPE cells should be more suitable for in vitro studies of the pathophysiology of retinochoroidal diseases.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"496","subitem_subject_scheme":"NDC"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"23791995"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山下, 高明","creatorNameLang":"ja"},{"creatorName":"YAMASHITA, Takehiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"42047","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000070404514","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"23791995.pdf","filesize":[{"value":"219.4 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"23791995.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/8655/files/23791995.pdf"},"version_id":"1aac9ba1-bda5-4693-972d-3cba85e58888"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"眼薬理学","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"硝子体注射","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"網膜色素上皮","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"TNF-α","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"薬物反応性","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"硝子体内投与薬物の眼組織障害の研究と臨床応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"硝子体内投与薬物の眼組織障害の研究と臨床応用","subitem_title_language":"ja"},{"subitem_title":"Eye tissue damage and clinical application of intra vitreous injection","subitem_title_language":"en"}]},"item_type_id":"8","owner":"18","path":["83"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-10-01"},"publish_date":"2013-10-01","publish_status":"0","recid":"8655","relation_version_is_last":true,"title":["硝子体内投与薬物の眼組織障害の研究と臨床応用"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-06-10T05:23:25.737650+00:00"}