{"created":"2023-07-25T08:09:08.968785+00:00","id":8660,"links":{},"metadata":{"_buckets":{"deposit":"9ef3f841-b4b7-4c89-ae68-6e8cee4ddfaa"},"_deposit":{"created_by":3,"id":"8660","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"8660"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00008660","sets":[]},"author_link":["118019","118017","118018","118020","6517","6518"],"item_8_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"A role of extracellular histones as mediators of death in sepsis"}]},"item_8_alternative_title_19":{"attribute_name":"タイトルよみ","attribute_value_mlt":[{"subitem_alternative_title":"ハイケツショウ ノ シンキ メディエーター サイボウガイ ヒストン ノ ダイナミズム ノ カイセキ"}]},"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2013-05-31"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2012-2012年度科学研究費助成事業(科学研究費補助金(挑戦的萌芽研究)研究成果報告書　課題番号：24659797　研究代表者：伊藤隆史(鹿児島大学・大学院医歯学総合研究科・特任講師)","subitem_description_type":"Other"},{"subitem_description":"(1) 血中ヒストンH3濃度を測定する方法の開発に取り組み、10-1000ng/mL のレンジで検出可能なELISA 系を確立した。(2) このELISA系を用いて血中のヒストンH3濃度を測定したところ、健康な人では測定した全例が0ng/mLだったのに対し、敗血症・DIC患者では0-1464(中央値:19.2)ng/mLと有意に高値を示した。また、生存例と比べ、死亡例では高値を示す傾向にあった。(3)マウスの尾静脈からヒストンを静注して病態解析を行ったところ、血栓塞栓症のほか、致死的不整脈を引き起こし、心停止に至ることが判明した。これらのことから、敗血症の際には、核内タンパク質ヒストンが細胞外に放出されて血中濃度が高まり、これによって血栓症や心筋傷害が誘発される可能性が考えられた。","subitem_description_type":"Other"},{"subitem_description":"We developed a sandwich ELISA to quantify serum or plasma histone H3 levels. The working range of this ELISA was 10-1000  g/L. Histone H3 was not detectable in plasma of healthy volunteers, but significant levels were observed in patients with sepsis and DIC. These levels were higher in non-survivors compared to survivors. Extracellular histones triggered platelet aggregation, leading to thrombotic occlusion of pulmonary capillaries and subsequent right-sided heart failure in mice. These mice displayed signs of DIC, including thrombocytopenia, prolonged prothrombin time, decreased fibrinogen, fibrin deposition in capillaries, and bleeding symptoms. These findings indicate that histones are released into the extracellular space during sepsis and act as a trigger for fatal thrombotic disorders.","subitem_description_type":"Other"}]},"item_8_full_name_2":{"attribute_name":"著者よみ","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"イトウ, タカシ"}]},{"nameIdentifiers":[{}],"names":[{"name":"マルヤマ, イクロウ"}]}]},"item_8_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"ITO, Takashi"}]},{"nameIdentifiers":[{}],"names":[{"name":"MARUYAMA, Ikuro"}]}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"494.27","subitem_subject_scheme":"NDC"}]},"item_8_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_8_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"24659797"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"伊藤, 隆史"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"丸山, 征郎"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"24659797.pdf","filesize":[{"value":"239.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"24659797.pdf","url":"https://ir.kagoshima-u.ac.jp/record/8660/files/24659797.pdf"},"version_id":"6dd36a69-a92e-477b-97ea-6eaec6802691"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"集中治療医学","subitem_subject_scheme":"Other"},{"subitem_subject":"敗血症","subitem_subject_scheme":"Other"},{"subitem_subject":"DIC","subitem_subject_scheme":"Other"},{"subitem_subject":"炎症","subitem_subject_scheme":"Other"},{"subitem_subject":"血栓","subitem_subject_scheme":"Other"},{"subitem_subject":"DAMPs","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"敗血症の新規メディエーター\"細胞外ヒストン\"のダイナミズムの解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"敗血症の新規メディエーター\"細胞外ヒストン\"のダイナミズムの解析"}]},"item_type_id":"8","owner":"3","path":["80"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-10-01"},"publish_date":"2013-10-01","publish_status":"0","recid":"8660","relation_version_is_last":true,"title":["敗血症の新規メディエーター\"細胞外ヒストン\"のダイナミズムの解析"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-12-25T07:18:51.046988+00:00"}