{"created":"2023-07-25T08:09:24.539390+00:00","id":8995,"links":{},"metadata":{"_buckets":{"deposit":"4ede972e-11e8-43ed-ae17-6a06a060c3cc"},"_deposit":{"created_by":18,"id":"8995","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"8995"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00008995","sets":["57:80"]},"author_link":["28013"],"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-06-17","subitem_date_issued_type":"Collected"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2012-2013年度科学研究費助成事業（若手研究（B））研究成果報告書　課題番号：24792003　研究代表者：富田和男（鹿児島大学・医歯（薬）学総合研究科・助教）","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"細胞死などに有意な変化を検出できない0.1mGy-100mGyといったごく低線量の放射線照射による細胞応答について、定量PCR法を用いて遺伝子発現変化を調べた。その結果、神経細胞芽腫NB-1細胞において、p53やMnSODなどの遺伝子発現が有意に変化することが明らかとなった。また、ミトコンドリアDNA突然変異を従来のRFLP法とSSCP法、および新しい方法であるTaqMan probeを用いた方法で比較検討した。その結果、TaqMan probeを用いた方法が、最も簡便に、かつ従来法より再現性良く突然変異を検出できることがわかった。","subitem_description_language":"ja","subitem_description_type":"Other"},{"subitem_description":"The cell response of low dose (0.1-100mGy) irradiation was investigated. Quantitative PCR was carried out whether specific gene expression change or not. As a result, 0.1 to 100mGy irradiation, that dose do not affect apoptosis ratio significantly, cause specific gene expression change such as p53, MnSOD in neuroblastoma cell line NB-1. Furthermore, to detect mitochondria DNA mutation, RELP, SSCP and TaqMan based QPCR was performed. As a result, The TaqMan probe method was the easiest and most reproductive among them.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"492","subitem_subject_scheme":"NDC"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"24792003"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"富田, 和男","creatorNameLang":"ja"},{"creatorName":"TOMITA, Kazuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"28013","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000060347094","nameIdentifierScheme":"NRID","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"24792003_富田和男.pdf","filesize":[{"value":"550.0 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"24792003_富田和男.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/8995/files/24792003_富田和男.pdf"},"version_id":"cc7ceb5a-6586-4396-92e2-d8af86c8ce87"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"病態科学系歯学","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"歯科放射線学","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"低線量放射線による細胞の放射線適応応答メカニズムの解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"低線量放射線による細胞の放射線適応応答メカニズムの解明","subitem_title_language":"ja"},{"subitem_title":"Analysis of Cell response by low dose irradiation","subitem_title_language":"en"}]},"item_type_id":"8","owner":"18","path":["80"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-05-19"},"publish_date":"2015-05-19","publish_status":"0","recid":"8995","relation_version_is_last":true,"title":["低線量放射線による細胞の放射線適応応答メカニズムの解明"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-05-24T00:24:43.295097+00:00"}