@techreport{oai:ir.kagoshima-u.ac.jp:00009008,
 author = {中村, 典史},
 month = {2016-10-28},
 note = {2011-2013年度科学研究費助成事業（基盤研究（C））研究成果報告書　課題番号：23592929　研究代表者：中村典史（鹿児島大学・医歯（薬）学総合研究科・教授）, 口腔粘膜上皮細胞由来の不死化細胞株（MOE-1）と濾胞型エナメル上皮腫細胞由来の不死化細胞株（AM-3）を作成し、既存の網状型エナメル上皮腫由来不死化細胞株AM-1と生物学的特性を比較した。AM-3はAm-1と類似の増殖態度、上皮系マーカーの発現を呈した。AM-1, AM-3はMOE-1よりMMP-2, -9の発現、Wnt関連遺伝子Wnt-5aやその受容体の発現が多かった。また、AM-3ではWnt-3a刺激によってMMP-9の発現が上昇することが観察された。これらのことから、エナメル上皮腫の局所浸潤性は、Wntシグナル経路を介したMMPの発現によって制御されていることが示唆された。, Immortalized epithelial cell line derived from oral mucosa  (MOE-1), as control, and immortalized ameloblastoma cell line derived from follicular-type ameloblastoma (AM-3) were established and their biological characteristics were compared to  AM-1, previously established immortalized ameloblastoma cells derived from prexiform-type ameloblastoma. AM-1 and AM-3 demonstrated positive reaction to the epithelial markers and negative to the mesenchymal one maintaining the morphological characteristics of the epithelial cell. Expressions of MMP-2 and MMP-9 were significantly higher in  AM-1 and AM-3 than  MOE-1. Wnt-5a and Wnt signaling receptors, LRP and Frizzled, of both  AM-1 and AM-3 were higher than  MOE-1 in the mRNA level. Furthermore, expression and activity of MMP-9 in AM-3 increased dose- and time-dependently when stimulated with Wnt-3a protein. These results suggested that local invasiveness of ameloblastoma may be regulated by MMPs expression through the Wnt signaling pathway.},
 title = {エナメル上皮腫による骨破壊・浸潤に関わる細胞間シグナルの解明と治療戦略への展開},
 year = {}
}