{"created":"2023-07-25T08:09:25.107486+00:00","id":9008,"links":{},"metadata":{"_buckets":{"deposit":"41c79ff1-9363-4d0c-acfd-21c910040f6f"},"_deposit":{"created_by":3,"id":"9008","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9008"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00009008","sets":[]},"author_link":["118033","118034","14913"],"item_8_alternative_title_18":{"attribute_name":"別言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Molecular analysis on the intercellular signaling relating to the bone destruction of ameloblastoma"}]},"item_8_alternative_title_19":{"attribute_name":"タイトルよみ","attribute_value_mlt":[{"subitem_alternative_title":"エナメル ジョウヒシュ ニヨル コツ ハカイ・ シンジュン ニ カカワル サイボウカン シグナル ノ カイメイ ト チリョウ センリャク エノ テンカイ"}]},"item_8_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2014-06-06"}]},"item_8_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"2011-2013年度科学研究費助成事業（基盤研究（C））研究成果報告書　課題番号：23592929　研究代表者：中村典史（鹿児島大学・医歯（薬）学総合研究科・教授）","subitem_description_type":"Other"},{"subitem_description":"口腔粘膜上皮細胞由来の不死化細胞株（MOE-1）と濾胞型エナメル上皮腫細胞由来の不死化細胞株（AM-3）を作成し、既存の網状型エナメル上皮腫由来不死化細胞株AM-1と生物学的特性を比較した。AM-3はAm-1と類似の増殖態度、上皮系マーカーの発現を呈した。AM-1, AM-3はMOE-1よりMMP-2, -9の発現、Wnt関連遺伝子Wnt-5aやその受容体の発現が多かった。また、AM-3ではWnt-3a刺激によってMMP-9の発現が上昇することが観察された。これらのことから、エナメル上皮腫の局所浸潤性は、Wntシグナル経路を介したMMPの発現によって制御されていることが示唆された。","subitem_description_type":"Other"},{"subitem_description":"Immortalized epithelial cell line derived from oral mucosa  (MOE-1), as control, and immortalized ameloblastoma cell line derived from follicular-type ameloblastoma (AM-3) were established and their biological characteristics were compared to  AM-1, previously established immortalized ameloblastoma cells derived from prexiform-type ameloblastoma. AM-1 and AM-3 demonstrated positive reaction to the epithelial markers and negative to the mesenchymal one maintaining the morphological characteristics of the epithelial cell. Expressions of MMP-2 and MMP-9 were significantly higher in  AM-1 and AM-3 than  MOE-1. Wnt-5a and Wnt signaling receptors, LRP and Frizzled, of both  AM-1 and AM-3 were higher than  MOE-1 in the mRNA level. Furthermore, expression and activity of MMP-9 in AM-3 increased dose- and time-dependently when stimulated with Wnt-3a protein. These results suggested that local invasiveness of ameloblastoma may be regulated by MMPs expression through the Wnt signaling pathway.","subitem_description_type":"Other"}]},"item_8_full_name_2":{"attribute_name":"著者よみ","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"ナカムラ, ノリフミ"}]}]},"item_8_full_name_3":{"attribute_name":"別言語の著者","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"NAKAMURA, Norifumi"}]}]},"item_8_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_8_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"493","subitem_subject_scheme":"NDC"}]},"item_8_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_8_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_8_text_41":{"attribute_name":"科研費番号 ","attribute_value_mlt":[{"subitem_text_value":"23592929"}]},"item_8_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中村, 典史"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"23592929_中村典史.pdf","filesize":[{"value":"559.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"23592929_中村典史.pdf","url":"https://ir.kagoshima-u.ac.jp/record/9008/files/23592929_中村典史.pdf"},"version_id":"65cfd509-969e-4231-a92f-e32704e6b533"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"口腔外科一般","subitem_subject_scheme":"Other"},{"subitem_subject":"エナメル上皮腫","subitem_subject_scheme":"Other"},{"subitem_subject":"歯原性腫瘍","subitem_subject_scheme":"Other"},{"subitem_subject":"Wntシグナル","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"エナメル上皮腫による骨破壊・浸潤に関わる細胞間シグナルの解明と治療戦略への展開","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"エナメル上皮腫による骨破壊・浸潤に関わる細胞間シグナルの解明と治療戦略への展開"}]},"item_type_id":"8","owner":"3","path":["80"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-22"},"publish_date":"2015-05-22","publish_status":"0","recid":"9008","relation_version_is_last":true,"title":["エナメル上皮腫による骨破壊・浸潤に関わる細胞間シグナルの解明と治療戦略への展開"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-12-25T07:19:04.785830+00:00"}