@article{oai:ir.kagoshima-u.ac.jp:00009190,
 author = {Furusawa, Gou and Yoshikawa, Takeshi and Takano, Yoshitaka and Mise, Kazuyuki and Furusawa, Iwao and Okuno, Tetsuro and Sakata, Taizo},
 issue = {10},
 journal = {Canadian journal of microbiology},
 month = {},
 note = {The cytoplasmic fibril structures of Saprospira sp. strain SS98-5 grown on a low-nutrient agar medium were
purified from cell lysates treated with Triton X-100 and were observed by electron microscopy to be about 7 nm in
width and 200-300 nm in length. SDS-PAGE of the fibril structures exhibited a single protein band with a molecular
mass of 61 kDa. A Saprospira cytoplasmic fibril protein (SCFP), which is a subunit of the fibril structures, was di
gested with trypsin to oligopeptides and analyzed for amino acid sequences. A partial nucleotide sequence of the SCFP
gene was determined after PCR using primers designated from the amino acid sequences of the oligopeptides. SCFP
gene including DNA fragments were detected by Southern hybridization using the PCR product for an SCFP gene as a
probe and were cloned to determine whole nucleotide sequences. The SCFP gene indicated relatively higher similarity
to conserved hypothetical phage tail sheath proteins. A Western immunoblotting analysis showed that SCFP was signif
icantly expressed in gliding cells as compared with nongliding cells. The above findings with the previously reported
results suggest that the cytoplasmic fibril structures are possibly related to the gliding motility of Saprospira sp. strain SS98-5.},
 pages = {875--880},
 title = {Characterization of cytoplasmic fibril structures found in gliding cells of Saprospira sp.},
 volume = {51},
 year = {2005}
}