{"created":"2023-07-25T08:09:33.972777+00:00","id":9201,"links":{},"metadata":{"_buckets":{"deposit":"b868f2d0-59ae-4947-a9f5-df7a2ee127f7"},"_deposit":{"created_by":18,"id":"9201","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"9201"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00009201","sets":["228:274:4795","64:65"]},"author_link":[],"item_7_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-03-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"29","bibliographicPageStart":"23","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"鹿児島大学歯学部紀要","bibliographic_titleLang":"ja"}]}]},"item_7_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"1996-03-25","subitem_date_issued_type":"Issued"}]},"item_7_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Mutans streptococci produce water-insoluble-glucan synthetase (glucosyltransfer-ase- I ), which is a pathogenic factor causing dental caries. The glucosyltransferase-I gene was isolated from Streptococcs sobrirtus ATCC33478^T and analyzed. The analysis showed that the gene existed in the Pst I fragment (6838 bp) of the S. sobrinus chromosome. The data of the DNA sequence showed that one open reading frame (DNA position, 1033-5805) coded 1590 amino acids, ribosome binding site (RBS, AGGAGGA) and promoter sequences (TTGTAA for -35,TAAAAT for -10) were located prior to the coding region, and two termination sequences were found after TAA stop codon. The amino-acid sequence indicated that the enzyme molecular size was 176,049,thirty-eight amino-acid residues from N terminus was a signal peptide for the enzyme secretion, and DSIRVDAVD (amino-acid position, 442-450) was a binding site of glucose-residue of sucrose. In the C-terminal one third of the sequence 71 glysine residues constructed nine G-repeat units. That C-terminal region is known as a glucan-binding region.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_7_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学","subitem_publisher_language":"ja"},{"subitem_publisher":"Kagoshima University","subitem_publisher_language":"en"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0389-7834","subitem_source_identifier_type":"PISSN"}]},"item_7_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN0035442X","subitem_source_identifier_type":"NCID"}]},"item_7_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"497","subitem_subject_scheme":"NDC"}]},"item_7_subject_20":{"attribute_name":"NIIsubject","attribute_value_mlt":[{"subitem_subject":"歯学","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_7_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"佐藤, 節子","creatorNameLang":"ja"},{"creatorName":"SATO, Setsuko","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"KJ00000165798.pdf","filesize":[{"value":"603.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000165798.pdf","objectType":"fulltext","url":"https://ir.kagoshima-u.ac.jp/record/9201/files/KJ00000165798.pdf"},"version_id":"f88a8e1b-f683-4cb2-9d73-6a854c2073f9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Mutans streptococci","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptococcus sobrinus ATCC33478^T","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"water-insoluble-glucan synthetase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA sequence","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"amino-acid sequence","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ミュータンス連鎖球菌の非水溶性多糖合成酵素遺伝子のDNA塩基配列とアミノ酸配列","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ミュータンス連鎖球菌の非水溶性多糖合成酵素遺伝子のDNA塩基配列とアミノ酸配列","subitem_title_language":"ja"},{"subitem_title":"DNA and amino-acid sequences of water-insoluble-glucan synthetase produced from Streptococcus sobrinus ATCC 33478^T","subitem_title_language":"en"}]},"item_type_id":"7","owner":"18","path":["65","4795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-04-28"},"publish_date":"2015-04-28","publish_status":"0","recid":"9201","relation_version_is_last":true,"title":["ミュータンス連鎖球菌の非水溶性多糖合成酵素遺伝子のDNA塩基配列とアミノ酸配列"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-01-26T07:09:27.046508+00:00"}