{"created":"2023-07-25T08:09:59.931951+00:00","id":9730,"links":{},"metadata":{"_buckets":{"deposit":"025f7af5-e7c8-489c-9b1b-988685221619"},"_deposit":{"created_by":3,"id":"9730","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9730"},"status":"published"},"_oai":{"id":"oai:ir.kagoshima-u.ac.jp:00009730","sets":["228:270:5677"]},"author_link":["95399","95400","95398"],"item_7_biblio_info_5":{"attribute_name":"収録雑誌名","attribute_value_mlt":[{"bibliographicPageEnd":"33","bibliographicPageStart":"25","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"Memoirs of the Faculty of Agriculture, Kagoshima University"}]}]},"item_7_date_6":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2005-02-20"}]},"item_7_description_4":{"attribute_name":"要約(Abstract)","attribute_value_mlt":[{"subitem_description":"Short-term or long-term space flight induces numerous biological stressors that affect several human physiological systems. The physical factors and their physiologic stressors cause several system dysfunctions. The incidence of tumor growth and carcinogenesis in microgravity is yet unknown. Hence, we investigated the effects of simulated microgravity on tumor growth and tumorigenicity using ground-based in vitro and in vivo models. Murine B16-F10 melanoma cells were cultured in a rotating wall vessel bioreactor (BIO), designed by NASA to simulate some aspects of microgravity, and a tissue culture flask (FL). We then measured cell growth, melanin production and apoptosis. After 48 hours of culture in FL and BIO, cells were inoculated subcutaneously in C57BL/6 mice syngeneic hosts for B16-F10 tumor cells. The growth of BIO cells decreased to 50% (p<0.01), and doubling time increased. Melanin production, a marker of differentiation, increased to 150% (p<0.05) in BIO. Flow cytometry analysis showed that there was increase in the percentage of apoptotic cells in the BIO when compared with that in the FL. When BIO cultured melanoma cells were inoculated subcutaneously in mice there was significant increase in tumorigenicity (p<.001) as compared to FL cells. Our results indicate that simulated microgravity may have altered the tumor cell characteristics and enhanced the invasive property. It is possible that the microgravity analog culture environment may have selected highly tumorigenic cells for survival despite the decreased overall growth in the microgravity analog.","subitem_description_type":"Other"}]},"item_7_publisher_23":{"attribute_name":"公開者・出版者","attribute_value_mlt":[{"subitem_publisher":"鹿児島大学"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"04530853","subitem_source_identifier_type":"ISSN"}]},"item_7_source_id_9":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00732376","subitem_source_identifier_type":"NCID"}]},"item_7_subject_15":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_7_subject_20":{"attribute_name":"NIIsubject","attribute_value_mlt":[{"subitem_subject":"農学","subitem_subject_scheme":"Other"}]},"item_7_text_24":{"attribute_name":"公開者よみ","attribute_value_mlt":[{"subitem_text_value":"カゴシマ ダイガク"}]},"item_7_text_25":{"attribute_name":"公開者別名","attribute_value_mlt":[{"subitem_text_value":"Kagoshima University"}]},"item_7_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TAGA, Masaki"}],"nameIdentifiers":[{"nameIdentifier":"95398","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KANOUCHI, Hiroaki"}],"nameIdentifiers":[{"nameIdentifier":"95399","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"OKA, Tatsuzo"}],"nameIdentifiers":[{"nameIdentifier":"95400","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-28"}],"displaytype":"detail","filename":"KJ00004161865.pdf","filesize":[{"value":"675.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00004161865.pdf","url":"https://ir.kagoshima-u.ac.jp/record/9730/files/KJ00004161865.pdf"},"version_id":"5e391be0-d132-43b0-b9b5-2ac3b3222576"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Tumor growth","subitem_subject_scheme":"Other"},{"subitem_subject":"tumorigenicity","subitem_subject_scheme":"Other"},{"subitem_subject":"microgravity","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effect of Simulated Microgravity on Murine Melanoma Cells : Altered Tumor Cell and Tumorigenicity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effect of Simulated Microgravity on Murine Melanoma Cells : Altered Tumor Cell and Tumorigenicity"}]},"item_type_id":"7","owner":"3","path":["44","5677"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-04-24"},"publish_date":"2007-04-24","publish_status":"0","recid":"9730","relation_version_is_last":true,"title":["Effect of Simulated Microgravity on Murine Melanoma Cells : Altered Tumor Cell and Tumorigenicity"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-01-30T05:14:47.128297+00:00"}